PXD039002

PXD039002 is an original dataset announced via ProteomeXchange.

Title	N-glycosite mapping in Brugia malayi female worms, male worms, and microfilariae
Description	N-linked glycosylation is a critical post translational modification of eukaryotic proteins. N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions. Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite. In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS. We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae. Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites. Our data identified 582 N-linked glycoproteins with 1273 N-glycosites. Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins. Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level. These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:58.445.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039002
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fana Mersha
SpeciesList	scientific name: Brugia malayi; NCBI TaxID: 6279;
ModificationList	amidated residue; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; 1x(18)O labeled deamidated L-asparagine; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 20:33:27	ID requested
1	2023-04-23 11:03:28	announced
⏵ 2	2023-11-14 08:59:58	announced	2023-11-14: Updated project metadata.

10.6019/PXD039002;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Glycoproteomics (B/D-HPP)

submitter keyword: LC-MSMS, N-glyco FASP,filarial parasite

Jeremy M. Foster
contact affiliation	Senior Scientist Protein Expression and Modification Division New England Biolabs
contact email	foster@neb.com
lab head
Fana Mersha
contact affiliation	New England Biolabs
contact email	mersha@neb.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039002
     1. Label: PRIDE project
     2. Name: N-glycosite mapping in Brugia malayi female worms, male worms, and microfilariae