PXD038992

PXD038992 is an original dataset announced via ProteomeXchange.

Title	Discovery of Novel Bilaterian Signaling Peptides Using Cone Snail Toxins
Description	Peptide hormones and neuropeptides form a diverse class of signaling molecules that control essential processes in animals. Despite several breakthroughs in peptide discovery, many signaling peptides remain undiscovered. Recently, we demonstrated the use of somatostatin-like toxins from cone snail venom to identify homologous signaling peptides in prey. Here, we demonstrate that this toxin-based approach can be systematically applied to the discovery of other unknown bilaterian signaling peptides. Using large sequencing datasets, we searched for homologies between cone snail toxins and putative peptides from several important model organisms representing the snails’ prey. We identified and confirmed expression of five toxin families that share strong similarities with previously unknown signaling peptides from mollusks and annelids. One of the peptides was also identified in rotifers, brachiopods, platyhelminths, and arthropods, and another was found to be structurally related to crustacean hyperglycemic hormone, a peptide not previously known to exist in Spiralia. Based on several lines of evidence we propose that these signaling peptides not only exist but serve important physiological functions. Finally, we propose that the discovery pipeline developed here can be more broadly applied to other systems in which one organism has evolved molecules to manipulate the physiology of another.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:58:27.834.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038992
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Koch
SpeciesList	scientific name: Conus marmoreus; NCBI TaxID: 42752;
ModificationList	amidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 20:11:52	ID requested
1	2023-08-18 05:04:31	announced
⏵ 2	2023-11-14 06:58:28	announced	2023-11-14: Updated project metadata.

10.6019/PXD038992;

submitter keyword: conotoxin, venom, hormone, neuropeptide

Helena Safavi-Hemami
contact affiliation	Department of Biochemistry, University of Utah, Salt Lake City, UT
contact email	helena.safavi@utah.edu
lab head
Thomas Koch
contact affiliation	University of Utah
contact email	thomas.lund.koch@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038992
     1. Label: PRIDE project
     2. Name: Discovery of Novel Bilaterian Signaling Peptides Using Cone Snail Toxins