PXD038968

PXD038968 is an original dataset announced via ProteomeXchange.

Title	Association of HMG20A to H2A.Z, PWWP2A and NuRD complex
Description	Specialized chromatin-binding proteins are required for DNA-based processes during development. We recently established PWWP2A as a direct histone variant H2A.Z interactor involved in mitosis and craniofacial development. Here, we identify the H2A.Z/PWWP2A-associated protein HMG20A as part of several chromatin-modifying complexes, including NuRD, andshow that it localizes to distinct genomic regulatory regions. Hmg20a depletion causes severe head and heart developmental defects in Xenopus laevis. Our data indicate that craniofacial malformations are caused by defects in neural crest cell (NCC) migration and cartilage formation. These developmental failures are phenocopied in Hmg20a-depleted mESCs, which show inefficient differentiation into NCCs and cardiomyocytes (CM). Consequently, loss of HMG20A, which marks open promoters and enhancers, results in chromatin accessibility changes and a striking deregulation of transcription programs involved in epithelial-mesenchymal transition (EMT) and differentiation processes. Collectively, our findings implicate HMG20A as part of the H2A.Z/PWWP2A/NuRD-axis and reveal it as a key modulator of intricate developmental transcription programs that guide the differentiation of NCCs and CMs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:01.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Reim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 06:59:45	ID requested
1	2023-03-11 11:16:56	announced
⏵ 2	2023-11-14 08:44:10	announced	2023-11-14: Updated project metadata.

Herchenr, ö, ther A, Gossen S, Friedrich T, Reim A, Daus N, Diegm, ü, ller F, Leers J, Sani HM, Gerstner S, Schwarz L, Stellmacher I, Szymkowiak LV, Nist A, Stiewe T, Borggrefe T, Mann M, Mackay JP, Bartkuhn M, Borchers A, Lan J, Hake SB, The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs. Nat Commun, 14(1):472(2023) [pubmed]

submitter keyword: AP-MS, HK cells,HMG20A, chromatin

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry, Dept. of Proteomics and Signal Transduction
contact email	mmann@biochem.mpg.de
lab head
Alexander Reim
contact affiliation	MPI of Biochemistry
contact email	alexanderreim1@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038968

PRIDE project URI

• PRIDE
  1. PXD038968
     1. Label: PRIDE project
     2. Name: Association of HMG20A to H2A.Z, PWWP2A and NuRD complex