PXD038941 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon Organoids |
Description | Human colon organoids were maintained in culture medium alone or exposed to lipopolysaccharide with a combination of three pro-inflammatory cytokines (tumor necrosis factor-a, interleukin-1b and interferon-g [LPS-cytokines]) to mimic the environment in the inflamed colon. Untreated organoids and those exposed to LPS-cytokines were concomitantly treated with a multi-mineral product that has previously been shown to improve barrier structure/function. The organoids were subjected to proteomic analysis to obtain a broad view of the protein changes induced by these interventions. In parallel, confocal fluorescence microscopy and trans-epithelial electrical resistance measurements were used to assess barrier structure/function. The LPS-cytokines altered expression of multiple proteins that influence innate immunity and promote inflammation. Most of these were unaffected by the multi-mineral intervention, though a subset of inflammation-related proteins including fibrinogen-b and -g chains, phospholipase A2 and SPARC was down-regulated in the presence of the multi-mineral intervention; another subset of proteins with anti-inflammatory, antioxidant or anti-microbial activity was up-regulated by multi-mineral treatment. When used alone, the multi-mineral intervention strongly up-regulated proteins that contribute to barrier formation and tissue strength. Concomitant treatment with LPS-cytokines did not inhibit barrier formation in response to the multi-mineral intervention. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:51:35.767.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Muhammad Aslam |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-19 05:20:41 | ID requested | |
1 | 2023-06-30 08:36:16 | announced | |
2 | 2023-11-14 06:59:26 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:51:36 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: LPS, proteomics, inflammation, cytokines, gut barrier, multi-mineral,Colon organoids |
Contact List
Muhammad Nadeem Aslam |
contact affiliation | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 |
contact email | mnaslam@umich.edu |
lab head | |
Muhammad Aslam |
contact affiliation | University of Michigan |
contact email | mnaslam@umich.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038941
- Label: PRIDE project
- Name: Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon Organoids