PXD038941

PXD038941 is an original dataset announced via ProteomeXchange.

Title	Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon Organoids
Description	Human colon organoids were maintained in culture medium alone or exposed to lipopolysaccharide with a combination of three pro-inflammatory cytokines (tumor necrosis factor-a, interleukin-1b and interferon-g [LPS-cytokines]) to mimic the environment in the inflamed colon. Untreated organoids and those exposed to LPS-cytokines were concomitantly treated with a multi-mineral product that has previously been shown to improve barrier structure/function. The organoids were subjected to proteomic analysis to obtain a broad view of the protein changes induced by these interventions. In parallel, confocal fluorescence microscopy and trans-epithelial electrical resistance measurements were used to assess barrier structure/function. The LPS-cytokines altered expression of multiple proteins that influence innate immunity and promote inflammation. Most of these were unaffected by the multi-mineral intervention, though a subset of inflammation-related proteins including fibrinogen-b and -g chains, phospholipase A2 and SPARC was down-regulated in the presence of the multi-mineral intervention; another subset of proteins with anti-inflammatory, antioxidant or anti-microbial activity was up-regulated by multi-mineral treatment. When used alone, the multi-mineral intervention strongly up-regulated proteins that contribute to barrier formation and tissue strength. Concomitant treatment with LPS-cytokines did not inhibit barrier formation in response to the multi-mineral intervention.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:51:35.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Aslam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 05:20:41	ID requested
1	2023-06-30 08:36:16	announced
2	2023-11-14 06:59:26	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:51:36	announced	2024-10-22: Updated project metadata.

10.3389/FCELL.2023.1132905;

submitter keyword: LPS, proteomics, inflammation, cytokines, gut barrier, multi-mineral,Colon organoids

Muhammad Nadeem Aslam
contact affiliation	Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109
contact email	mnaslam@umich.edu
lab head
Muhammad Aslam
contact affiliation	University of Michigan
contact email	mnaslam@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD038941

PRIDE project URI

• PRIDE
  1. PXD038941
     1. Label: PRIDE project
     2. Name: Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon Organoids