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PXD038941

PXD038941 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRed Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon Organoids
DescriptionHuman colon organoids were maintained in culture medium alone or exposed to lipopolysaccharide with a combination of three pro-inflammatory cytokines (tumor necrosis factor-a, interleukin-1b and interferon-g [LPS-cytokines]) to mimic the environment in the inflamed colon. Untreated organoids and those exposed to LPS-cytokines were concomitantly treated with a multi-mineral product that has previously been shown to improve barrier structure/function. The organoids were subjected to proteomic analysis to obtain a broad view of the protein changes induced by these interventions. In parallel, confocal fluorescence microscopy and trans-epithelial electrical resistance measurements were used to assess barrier structure/function. The LPS-cytokines altered expression of multiple proteins that influence innate immunity and promote inflammation. Most of these were unaffected by the multi-mineral intervention, though a subset of inflammation-related proteins including fibrinogen-b and -g chains, phospholipase A2 and SPARC was down-regulated in the presence of the multi-mineral intervention; another subset of proteins with anti-inflammatory, antioxidant or anti-microbial activity was up-regulated by multi-mineral treatment. When used alone, the multi-mineral intervention strongly up-regulated proteins that contribute to barrier formation and tissue strength. Concomitant treatment with LPS-cytokines did not inhibit barrier formation in response to the multi-mineral intervention.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:51:35.767.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMuhammad Aslam
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-19 05:20:41ID requested
12023-06-30 08:36:16announced
22023-11-14 06:59:26announced2023-11-14: Updated project metadata.
32024-10-22 05:51:36announced2024-10-22: Updated project metadata.
Publication List
10.3389/FCELL.2023.1132905;
Keyword List
submitter keyword: LPS, proteomics, inflammation, cytokines, gut barrier, multi-mineral,Colon organoids
Contact List
Muhammad Nadeem Aslam
contact affiliationDepartment of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109
contact emailmnaslam@umich.edu
lab head
Muhammad Aslam
contact affiliationUniversity of Michigan
contact emailmnaslam@umich.edu
dataset submitter
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Dataset FTP location
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Repository Record List
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