PXD038935

PXD038935 is an original dataset announced via ProteomeXchange.

Title	The structural basis of the multi-step allosteric activation of Aurora B kinase
Description	Aurora B, together with IN-box, the C-terminal part of INCENP, forms an enzymatic complex that ensures faithful cell division. The [Aurora B/IN-box] complex is activated by autophosphorylation in the Aurora B activation loop and in IN-box, but it is not clear how these phosphorylations activate the enzyme. We used a combination of experimental and computational studies to investigate the effects of phosphorylation on the molecular dynamics and structure of [Aurora B/IN-box]. In addition, we generated partially phosphorylated intermediates to analyze the contribution of each phosphorylation independently. We found that the dynamics of Aurora and IN-box are interconnected, and IN-box plays both positive and negative regulatory roles depending on the phosphorylation status of the enzyme complex. Phosphorylation in the activation loop of Aurora B occurs intramolecularly and prepares the enzyme complex for activation, but two phosphorylated sites are synergistically responsible for full enzyme activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:46:02.800.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dario Segura-Pena
SpeciesList	scientific name: Xenopus cf. laevis UF 185698; NCBI TaxID: 2664373;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 04:02:13	ID requested
1	2023-05-31 09:08:57	announced
2	2023-11-14 08:59:35	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:46:03	announced	2024-10-22: Updated project metadata.

10.7554/ELIFE.85328;

submitter keyword: Xenopus Laevis, HDX-MS,Aurora B

Nikolina Sekulic
contact affiliation	Centre for Molecular Medicine Norway
contact email	nikolina.sekulic@ncmm.uio.no
lab head
Dario Segura-Pena
contact affiliation	Researcher
contact email	dario.segura-pena@ncmm.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD038935

PRIDE project URI

• PRIDE
  1. PXD038935
     1. Label: PRIDE project
     2. Name: The structural basis of the multi-step allosteric activation of Aurora B kinase