PXD038931

PXD038931 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of male accessory gland secretions in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)
Description	In insects, male accessory gland proteins (ACPs) are important reproductive proteins secreted by male accessory glands (MAGs) of the internal male reproductive system. During mating, ACPs were transferred along with sperms inside female bodies and have a significant impact on the physiology of female reproduction. Under sexual selection pressures, the ACPs exhibit remarkably rapid and divergent evolution and varies from species to species. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major insect pest of cruciferous vegetables worldwide. The reproductive physiology on post-mating state of this species is still largely unknown, which is important for management of this pest. In this study, the ACPs transferred into females during mating were identified by using a tandem mass tags quantitative proteomic analysis. The MAGs were compared before and after mating immediately. In total, we identified 123 putative secreted ACPs, including most important physiological: regulators of proteolysis, transporters and protein export machinery, signal transduction and immunity. Comparing P. xylostella with other four insect ACPs, trypsins were the only ACPs detected in all insect species. This was the first time to identify and analyze ACPs in P. xylostella. Our results have provided an important list of putative secreted ACPs, and have set the stage for further explore functions of these putative proteins in P. xylostella reproduction.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:41:24.506.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lijuan Wu
SpeciesList	scientific name: Plutella xylostella (Diamondback moth) (Plutella maculipennis); NCBI TaxID: 51655;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 03:54:16	ID requested
1	2023-03-11 03:09:54	announced
⏵ 2	2023-11-14 08:41:27	announced	2023-11-14: Updated project metadata.

Wu LJ, Li F, Song Y, Zhang ZF, Fan YL, Liu TX, (Lepidoptera: Plutellidae). Insects, 14(2):(2023) [pubmed]

submitter keyword: Plutella xylostella

seminal fluid proteins

male accessory gland

accessory gland proteins

proteomics

Lijuan Wu
contact affiliation	College of Plant Protection, Northwest A&F University
contact email	wu_lijuan@nwafu.edu.cn
lab head
Lijuan Wu
contact affiliation	College of Plant Protection, Northwest A&F University
contact email	wu_lijuan@nwafu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038931
     1. Label: PRIDE project
     2. Name: Proteome analysis of male accessory gland secretions in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)