PXD038927

PXD038927 is an original dataset announced via ProteomeXchange.

Title	Staphylococcus aureus protein binding in human serum
Description	Overnight cultures of S. aureus were diluted to OD600 = 1 and 1 ml of culture was resuspended in 500 μl of either PBS, human plasma, or human serum, and incubated rotating at 37 °C for 30 m. Suspensions were washed 3x with PBS supplemented with 500 40% sucrose and 20 mM Sodium Azide. Cells were incubated with immobilized trypsin (ThermoFisher 20230), suspended in PBS supplemented with 500 40% sucrose and 20 mM Sodium Azide, at 37 °C for 2 h. cells were pelleted, and the supernatant containing protein fragments was analyzed by mass spectrometry to determine proteins.
HostingRepository	PRIDE
AnnounceDate	2023-04-14
AnnouncementXML	Submission_2023-04-14_11:56:13.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MarcusWittekind
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 03:45:13	ID requested
⏵ 1	2023-04-14 11:56:13	announced

Dataset with its publication pending

submitter keyword: S aureus, Human Serum Binding,Staphylococcus aureus

RonanCarroll
contact affiliation	Ohio University, Biological Sciences Department, Athens Ohio, United States
contact email	carrolr3@ohio.edu
lab head
MarcusWittekind
contact affiliation	Ohio University
contact email	mw064216@ohio.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD038927

PRIDE project URI

• PRIDE
  1. PXD038927
     1. Label: PRIDE project
     2. Name: Staphylococcus aureus protein binding in human serum