PXD038920

PXD038920 is an original dataset announced via ProteomeXchange.

Title	Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis
Description	Actin-related proteins (Arp) are classified according to their similarity to actin and are involved in diverse cellular processes. ACTL7B is a testis-specific Arp and highly conserved in rodents and primates. ACTL7B is specifically expressed in round and elongating spermatids during spermiogenesis. Here, we have generated an Actl7b-null allele in mice to unravel the role of ACTL7B in sperm formation. Male mice homozygous for the Actl7b-null allele (Actl7b-/-) were infertile, while heterozygous males (Actl7b+/-) were fertile. Severe spermatid defects such as detached acrosomes, disrupted membranes and failed elongation of the axoneme start to appear at spermiogenesis step 9 in Actl7b-/- mice, finally resulting in spermatogenic arrest. Abnormal spermatids, were degraded. Co-immunoprecipitation experiments identified interaction between ACTL7B and the LC8 dynein light chains DYNLL1 and DYNLL2, which are first detected in step 9 spermatids and mislocalized when ACTL7B is absent. Our data unequivocally establishes that mutations in ACTL7B are directly related to male infertility, pressing for additional research in men.
HostingRepository	PRIDE
AnnounceDate	2023-10-19
AnnouncementXML	Submission_2023-10-19_04:10:11.044.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038920
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	MarcSylvester
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 03:31:44	ID requested
⏵ 1	2023-10-19 04:10:11	announced

10.6019/PXD038920;

submitter keyword: Actl7b, LC8 light chains, actin-related proteins, mouse, infertility, spermatogenesis

Prof. Dr. HubertSchorle
contact affiliation	Department of Developmental Pathology and Department of Molecular Diagnostics Institute of Pathology Bonn Medical School Venusberg-Campus 1, Building 62 53127 Bonn
contact email	schorle@uni-bonn.de
lab head
MarcSylvester
contact affiliation	Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn
contact email	msylvest@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038920

PRIDE project URI

• PRIDE
  1. PXD038920
     1. Label: PRIDE project
     2. Name: Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis