PXD038891

PXD038891 is an original dataset announced via ProteomeXchange.

Title	Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway
Description	The propagation of intracellular T cell receptor (TCR) signals involved various adapter proteins that are important molecular switches to connect proteins together. The global characterization of changes in protein-protein interactions following genetic perturbations is critical to understand the reorganization of protein networks associated with the observed phenotypes. Here, by combining genome editing techniques and AP-MS analysis we determined and quantified the molecular reorganization of the SLP76 interactome resulting from the inactivation of each of the three GRB2-family adaptors. Our data define disruption context-specific and time-dependent networks that form around SLP76 after TCR stimulation, and selectively preserved or highly affected protein complexes that regulate T cell activation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:06.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 02:25:24	ID requested
1	2023-03-13 02:53:37	announced
⏵ 2	2023-11-14 09:00:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: AP-MS, T cell,SLP76, GRB2-family adaptors

Luc Camoin & Romain Roncagalli
contact affiliation	Marseille Protéomique, Inserm, CNRS, Aix-Marseille Université, Centre d'Immunology de Marseille-Luminy (CIML), Centre de Recherche en Cancérologie de Marseille (CRCM)
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038891

PRIDE project URI

• PRIDE
  1. PXD038891
     1. Label: PRIDE project
     2. Name: Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway