PXD038884

PXD038884 is an original dataset announced via ProteomeXchange.

Title	4D label free-based quantitative proteomic analysis of Eimeria tenella unsporulated oocysts and sporulated oocysts
Description	We performed 4D label free-based quantitative proteomic analysis of Eimeria tenella unsporulated oocysts and sporulated oocysts. Briefly, protein extraction was performed using liquid nitrogen grinding and sonication on ice in SDT buffer (4% SDS, 100 mM Tris-HCl, pH7.6). After trypsin digestion, the peptides were separated on Easy-nLC1000 liquid chromatograph (Thermo Fisher Scientific, Waltham, MA, USA) with a trap column (Thermo Fisher Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) and analytical column (Thermo Fisher Scientific Easy Column, 25 cm long, 75 μm inner diameter, 1.9 μm resin). LC-MS/MS analysis was conducted on a timsTOF Pro mass spectrometry. The resulting LC-MS/MS data files were processed with the MaxQuant software (version 1.5.3.17) for identification and quantitation analysis.
HostingRepository	iProX
AnnounceDate	2022-12-18
AnnouncementXML	Submission_2022-12-18_19:50:01.121.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qing Liu
SpeciesList	scientific name: Eimeria tenella; NCBI TaxID: 5802;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-18 19:49:40	ID requested
⏵ 1	2022-12-18 19:50:01	announced

Dataset with its publication pending

submitter keyword: Eimeria tenella, proteomic analysis, unsporulated oocysts, sporulated oocysts

Xingquan Zhu
contact affiliation	Shanxi Agricultural University
contact email	xingquanzhu1@hotmail.com
lab head
Qing Liu
contact affiliation	Shanxi Agricultural University
contact email	lqsxau@163.com
dataset submitter

iProX dataset URI