PXD038870

PXD038870 is an original dataset announced via ProteomeXchange.

Title	Tryptophan zipper hydrogels: bioactive self-healing antimicrobial matrices
Description	Natural materials can reversibly self-assemble into hierarchical networks with diverse structural and functional properties. Recreating these dynamic architectures using peptide-based synthetic materials has been an elusive goal, hindered by challenges in relating sequence to structure to function. Here we report on the de novo discovery of short peptides based on the “tryptophan zipper” (Trpzip) motif that self-assemble into hierarchically structured hydrogels. Trpzip hydrogels have a tunable modulus and show self-healing, stress relaxation, antimicrobial properties, and biocompatibility. The low yield point allows syringe extrusion with cytoprotection, and cell harvest with a flick of the wrist. Integrating a pendant cell adhesion motif promotes human intestinal organoid growth and differentiation, with polarity control and improved shape fidelity. Considering these unique characteristics, we anticipate Trpzip hydrogels will prove a versatile reagent for biotechnology and medicine.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:13:42.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abhishek Vijayan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-16 00:06:30	ID requested
1	2023-10-24 22:25:08	announced
2	2023-11-05 10:30:32	announced	2023-11-05: Updated project metadata.
3	2023-11-14 09:11:36	announced	2023-11-14: Updated project metadata.
⏵ 4	2023-11-14 09:13:44	announced	2023-11-14: Updated project metadata.

Nguyen AK, Molley TG, Kardia E, Ganda S, Chakraborty S, Wong SL, Ruan J, Yee BE, Mata J, Vijayan A, Kumar N, Tilley RD, Waters SA, Kilian KA, Hierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogels. Nat Commun, 14(1):6604(2023) [pubmed]

submitter keyword: Organoids, Matrigel,Hydrogel

Shafagh Waters
contact affiliation	School of Biomedical Sciences, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW, 2052, Australia
contact email	shafagh.waters@unsw.edu.au
lab head
Abhishek Vijayan
contact affiliation	UNSW Sydney
contact email	abhishek.kcvijayan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038870

PRIDE project URI

• PRIDE
  1. PXD038870
     1. Label: PRIDE project
     2. Name: Tryptophan zipper hydrogels: bioactive self-healing antimicrobial matrices