PXD038861
PXD038861 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass spectrometry based identification of similarities and differences in protein content of cutaneous melanoma cell lines and exosomes |
| Description | Intercellular transport of proteins mediated by extracellular vesicles (EVs) exosomes and ectosomes, is one of the factors facilitating carcinogenesis. Therefore, the research on protein cargo of melanoma-derived EVs may provide a better understanding of mechanisms related to melanoma progression and contribute to development of novel biomarkers. The data concerning the proteome of melanoma-derived EVs is very limited. We used the shotgun nanoLC-MS/MS approach to the profile protein content of primary (WM115, WM793) and metastatic (WM2664, WM1205Lu) cutaneous melanoma cell lines and exosomes. All cell lines secreted homogeneous populations of exosomes enriched in the common set of proteins. A total of 3514 and 1234 unique proteins were identified in melanoma cells and exosomes, respectively. Gene Ontology analysis showed that many of them were involved in cancer cell proliferation, migration, escape from apoptosis, epithelial mesenchymal transition and angiogenesis. The obtained results expand the knowledge about the role of selected proteins in the biology of exosomes, as well as their functional role in the development and progression of cutaneous melanoma. The results might also be used as a starting point for further studies exploring diagnostic and prognostic potential of exosomes. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-19 |
| AnnouncementXML | Submission_2024-11-19_05:00:33.643.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Urszula Jankowska |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Acetyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-12-15 07:01:47 | ID requested | |
| ⏵ 1 | 2024-11-19 05:00:34 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cancer, extracellular vesicles, exosomes, melanoma, proteomics |
Contact List
| Malgorzata Przybylo | |
|---|---|
| contact affiliation | Department of Glycoconjugate Biochemistry, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University |
| contact email | malgorzata.przybylo@uj.edu.pl |
| lab head | |
| Urszula Jankowska | |
| contact affiliation | Jagiellonian University |
| contact email | urszula.jankowska@uj.edu.pl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000090907/ |
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