PXD038854

PXD038854 is an original dataset announced via ProteomeXchange.

Title	Cardiomyocytes regulate cardiac fibroblast activation and oxidative stress via CK2 signaling
Description	Secretome-mediated signaling from human iPSC-derived cardiomyocytes (TGFβ induced dysfunction) to primary human cardiac fibroblasts was investigated to identify downstream regulators of fibrosis. Quantitative proteomic profiling revealed a dynamic reprogramming of fibroblast global proteome, with dysregulation of proteins implicated in extracellular matrix (ECM) remodelling, cytoskeleton organization, lysosome function, and oxidoreductase- and kinase activity. Protein modification-focused processing analyses of mass spectrometry proteome data further highlight phospho-proteome alterations in pro-fibrotic pathways regulated by various kinases (CK2, CDK1, CDK2, MAPK1, PRKACA, PRKG1). We verified upregulated casein kinase 2 (CK2) substrate levels in secretome-treated fibroblasts, and pharmacological inhibition of CK2 using TBB (4,5,6,7-Tetrabromobenzotriazole) significantly abrogated reactive oxygen species’ levels and activation state (SMA+).
HostingRepository	PRIDE
AnnounceDate	2025-02-11
AnnouncementXML	Submission_2025-02-11_14:23:42.667.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	david greening
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-14 22:12:24	ID requested
⏵ 1	2025-02-11 14:23:43	announced

Claridge B, Rai A, Lees JG, Fang H, Lim SY, Greening DW, Cardiomyocyte intercellular signalling increases oxidative stress and reprograms the global- and phospho-proteome of cardiac fibroblasts. J Extracell Biol, 2(12):e125(2023) [pubmed]

10.1002/jex2.125;

ProteomeXchange project tag: Cardiovascular (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: cardiac fibroblasts, cardiomyocytes,proteomics, CK2, signaling

David Greening
contact affiliation	Baker Heart & Diabetes Institute
contact email	david.greening@baker.edu.au
lab head
david greening
contact affiliation	Baker Heart and Diabetes Inst.
contact email	david.greening@baker.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD038854

PRIDE project URI

• PRIDE
  1. PXD038854
     1. Label: PRIDE project
     2. Name: Cardiomyocytes regulate cardiac fibroblast activation and oxidative stress via CK2 signaling