PXD038847

PXD038847 is an original dataset announced via ProteomeXchange.

Title	Adaptation of Lacticaseibacillus rhamnosus CM MSU 529 to aerobic and respiratory growth: a proteomic approach
Description	Lacticaseibacillus rhamnosus CM MSU 529 is the O2-tolerant facultative homofermentative lactic acid bacteria realizing respiratory metabolism when grown in a supplemented with hemin and vitamin K2 nutrient medium. This study describes the effect of aerobic and respiratory conditions on biomass and proteome of strain CM MSU 529 grown in a batch culture. Aeration caused the induction of the biosynthesis of 43 proteins, while 14 proteins were down-regulated as detected by label-free LC-MS/MS. Up-regulated proteins are involved in oxygen consumption (Pox, LctO, pyridoxine 5'-phosphate oxidase), xylulose-5-phosphate conversion (Xfp), pyruvate metabolism (PdhD, AlsS, AlsD), reactive oxygen species (ROS) elimination (Tpx, TrxA, Npx), general stress response (GroES, PfpI, universal stress protein, YqiG), antioxidant production (CysK, DkgA), pyrimidine metabolism (CarA, CarB, PyrE, PyrC, PyrB, PyrR), oligopeptide transport and metabolism (OppA, PepO), maturation and stability of ribosomal subunits (RbfA, VicX). Down-regulated proteins participate in ROS defense (AhpC), citrate and pyruvate consumption (CitE, PflB), oxaloacetate production (AvtA), arginine synthesis (ArgG), amino acid transport (GlnQ), and deoxy-nucleoside biosynthesis (RtpR). Overproduction of purine biosynthesis enzyme (PurE) distinguished cells grown under respiratory conditions from cells grown under aerobiosis. The data obtained shed light on mechanisms providing O2-tolerance and adaptation to aerobic/respiratory conditions in strain CM MSU 529.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:35.548.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joerg Kahnt
SpeciesList	scientific name: Lacticaseibacillus rhamnosus LRHMDP2; NCBI TaxID: 1203258;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-14 22:05:19	ID requested
1	2023-03-11 10:36:35	announced
⏵ 2	2023-11-14 08:43:37	announced	2023-11-14: Updated project metadata.

Dinarieva TY, Klimko AI, Kahnt J, Cherdyntseva TA, Netrusov AI, CM MSU 529 to Aerobic Growth: A Proteomic Approach. Microorganisms, 11(2):(2023) [pubmed]

submitter keyword: Lacticaseibacillus rhamnosus

aerobic conditions

respiratory conditions

oxidative stress

pyruvate oxidase

pyridoxine 5'-phosphate oxidase

NADH peroxidase

acetolactate synthase

Timo Glatter
contact affiliation	Core Facility for Mass spectrometry & Proteomics, Max Planck Institut Marburg, Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Joerg Kahnt
contact affiliation	Max Planc Institut für terrestrische Mikrobiologie
contact email	kahnt@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038847
     1. Label: PRIDE project
     2. Name: Adaptation of Lacticaseibacillus rhamnosus CM MSU 529 to aerobic and respiratory growth: a proteomic approach