PXD038840

PXD038840 is an original dataset announced via ProteomeXchange.

Title	Immunization with a heat-killed Prm1 deletion strain protects the host from Cryptococcus neoformans infection
Description	Systemic infection with Cryptococcus neoformans, a dangerous and contagious pathogen found throughout the world, frequently results in lethal cryptococcal pneumonia and meningoencephalitis, and no effective treatments of cryptococcosis are available. Here, we describe Prm1, a novel regulator of virulence in C. neoformans. C. neoformans prm1 cells exhibit extreme sensitivity to various environmental stress conditions. Furthermore, prm1 cells show deficiencies in the biosynthesis of chitin, chitosan, and mannoprotein, which in turn result in impairment of cell wall integrity. Treatment of mice with heat-killed prm1 cells was found to facilitate the host immunological defence against infection with wild-type C. neoformans. Further investigation demonstrated that prm1 cells strongly promote pulmonary production of interferon- and Th1 responses, leading to activation of macrophage M1 differentiation and inhibition of M2 polarization. Therefore, our findings suggest that C. neoformans Prm1 may be a viable target for the development of anti-cryptococcosis medications and, cells lacking Prm1 represent a promising candidate for a vaccine.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:33.668.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	超 李
SpeciesList	scientific name: Cryptococcus neoformans AD hybrid; NCBI TaxID: 192011;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-14 21:55:05	ID requested
1	2023-10-11 02:24:19	announced
2	2023-11-14 08:29:54	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:05:34	announced	2024-10-22: Updated project metadata.

10.1080/22221751.2023.2244087;

submitter keyword: Cryptococcus neoformans

MMP

cell wall

fungal vaccine

IFN-γ

macrophage polarization

drug targets.

Chao li
contact affiliation	College of Life and Health Sciences, Northeastern University
contact email	fMssop_li@163.com
lab head
超 李
contact affiliation	College of Life and Health Sciences, Northeastern University
contact email	fMssop_li@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038840

PRIDE project URI

• PRIDE
  1. PXD038840
     1. Label: PRIDE project
     2. Name: Immunization with a heat-killed Prm1 deletion strain protects the host from Cryptococcus neoformans infection