PXD038836 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Elucidating Fibroblast Growth Factor-induced kinome dynamics using targeted mass spectrometry and dynamic modeling |
| Description | Fibroblast growth factors (FGFs) are paracrine or endocrine signaling proteins that when activated by their ligands, can elicit a wide range of healthy and disease-related processes such as cell proliferation and the epithelial-to-mesenchymal transition (EMT). The detailed molecular pathway dynamics that coordinate these responses have remained unclear. To elucidate and monitor these dynamics we stimulated MCF-7 breast cancer cells with either FGF2, 3, 4, 10, or 19. Following activation of the receptor, we quantified the kinase activity dynamics of 44 kinases using a targeted mass spectrometry assay. Our system-wide kinase activity data, supplemented with (phopsho)proteomics data, reveal ligand-dependent distinct pathway dynamics, elucidate the involvement of not earlier reported kinases such as MARK, and revise some of the pathway effects on biological outcomes. In addition, logic-based dynamic modeling of the kinome dynamics further verifies the biological goodness-of-fit of the predicted models and reveals tight regulation of the RAF kinase family. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:51:39.686.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tim Veth |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-12-14 21:51:43 | ID requested | |
| 1 | 2023-07-02 04:26:38 | announced | |
| 2 | 2023-11-14 09:01:45 | announced | 2023-11-14: Updated project metadata. |
| ⏵ 3 | 2024-10-22 05:51:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.1016/j.mcpro.2023.100594; |
| Veth TS, Francavilla C, Heck AJR, Altelaar M, Elucidating Fibroblast Growth Factor-Induced Kinome Dynamics Using Targeted Mass Spectrometry and Dynamic Modeling. Mol Cell Proteomics, 22(8):100594(2023) [pubmed] |
Keyword List
| submitter keyword: Phosphoproteomics |
| kinase |
| targeted mass spectrometry |
| kinase dynamics |
| fibroblast growth factors |
| dynamic modeling |
| fibroblast growth factor receptor |
| breast cancer. |
Contact List
| Maarten Altelaar |
|---|
| contact affiliation | 1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht, 3584 CH, The Netherlands 2. Netherlands Proteomics Center, Padualaan 8, Utrecht, 3584 CH, The Netherlands |
| contact email | m.altelaar@uu.nl |
| lab head | |
| Tim Veth |
|---|
| contact affiliation | Utrecht University |
| contact email | t.s.veth@uu.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD038836 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038836
- Label: PRIDE project
- Name: Elucidating Fibroblast Growth Factor-induced kinome dynamics using targeted mass spectrometry and dynamic modeling
to receive all new ProteomeXchange dataset release announcements!
