PXD038835

PXD038835 is an original dataset announced via ProteomeXchange.

Title	Triggering the polyurethane and polyethylene-degrading potential of filamentous fungi secretomes
Description	In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based PU and LDPE, using Impranil and a mixture of long-chain alkanes, not only as sole carbon sources but also as enzyme indicators for polymer degradation. The agar plate screening revealed 3 fungal strains belonging to Fusarium and Aspergillus genera, which were further cultured in presence of the same carbon sources, and their secretome was utilized for polymer degradation. Especially for ether-based PU, the secreted proteins of a Fusarium species reduced the weight and the molecular weight of the sample by 24.5 and 20.4%, respectively, while at the same time the secretome of an Aspergillus species caused changes in the molecular structure of LDPE. The proteomic analysis followed proved that the enzymes induced in presence of Impranil can selectively degrade the urethane bond of ether-based PU, a discovery that offers a new alternative in PU waste treatment. Meanwhile, the mechanism of LDPE degradation was not completely understood, although the presence of oxidative enzymes probably accompanied by reactive oxygen species, could be the main factors contributing to polymer functionalization.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:12.533.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038835
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romanos Siaperas
SpeciesList	scientific name: Aspergillus transmontanensis; NCBI TaxID: 1034304; scientific name: Fusarium oxysporum; NCBI TaxID: 5507;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-14 21:51:18	ID requested
1	2023-04-27 07:14:04	announced
⏵ 2	2023-11-14 08:45:14	announced	2023-11-14: Updated project metadata.

10.6019/PXD038835;

Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelic B, Nikodinovic-Runic J, Topakas E, Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. Environ Pollut, 325():121460(2023) [pubmed]

submitter keyword: Label free, Secretome, Fungi,LC-MSMS, Plastics

Evangelos Topakas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	vtopakas@chemeng.ntua.gr
lab head
Romanos Siaperas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	roman_siap@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD038835

PRIDE project URI

• PRIDE
  1. PXD038835
     1. Label: PRIDE project
     2. Name: Triggering the polyurethane and polyethylene-degrading potential of filamentous fungi secretomes