PXD038823

PXD038823 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis YchF1 binds ribosomal protein S7 to regulate translation at the heavy polysome
Description	Protein synthesis plays a pivotal role in determining the overall gene expression profile of the cell. The involvement of YchFs in translation has been hinted at in previous studies on bacterial, protozoan, and mammalian cells. In plant, YchF has been characterized as an unconventional P-loop GTPase that interacts with various ligands including rRNA. Here, using the model organism, Arabidopsis, we demonstrated the significance of ribosomal protein S7 (AtRPS7) binding by AtYchF1 on salt sensitivity. Polysome profiling suggested that AtYchF1 regulates translational efficiency in an AtRPS7-binding-depdenent manner. Translatomic and proteomic analyses together indicated that AtRPS7 regulates translation events at the heavy polysome through interacting with AtRPS7. A docking model was constructed and validated by site-directed mutagenesis and in vitro pull-down experiments to illustrate the molecular interactions between AtYchF1 and AtRPS7. Our study offers a structural framework for understanding the molecular basis of YchF proteins on translational regulation and stress responses.
HostingRepository	PRIDE
AnnounceDate	2026-08-21
AnnouncementXML	Submission_2026-08-20_20:50:00.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sau-Shan Cheng
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 23:23:25	ID requested
⏵ 1	2026-08-20 20:50:00	announced

10.1016/J.XPLC.2026.101775;

submitter keyword: TMT labelling,Arabidopsis

Hon-Ming LAM
contact affiliation	School of Life Sciences and Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong SAR, China
contact email	honming@cuhk.edu.hk
lab head
Sau-Shan Cheng
contact affiliation	Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China
contact email	1155063073@link.cuhk.edu.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038823
     1. Label: PRIDE project
     2. Name: Arabidopsis YchF1 binds ribosomal protein S7 to regulate translation at the heavy polysome