PXD038818

PXD038818 is an original dataset announced via ProteomeXchange.

Title	D2O proteome dynamics in FSHD vs UASb myoblasts
Description	Proteomic studies in facioscapulohumeral muscular dystrophy (FSHD) could offer new insight to disease mechanisms underpinned by post-transcriptional processes. We used stable isotope (deuterium oxide; D2O) labelling and peptide mass spectrometry to investigate the abundance and turnover rates of proteins in cultured muscle cells from 2 individuals affected by FSHD and their unaffected siblings (UASb). We measured the abundance of 4483 proteins and the turnover rate of 2324 proteins in each (n = 4) myoblast sample. FSHD myoblasts exhibited a greater abundance but slower turnover rate of subunits of mitochondrial respiratory complexes and mitochondrial ribosomal proteins, which may indicate an accumulation of ‘older’ less viable mitochondrial proteins in myoblasts from individuals affected by FSHD. Our results highlight the importance of post-transcriptional processes and protein turnover in FSHD pathology and provide a resource for the FSHD research community to explore this burgeoning aspect of FSHD.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:40.627.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038818
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jatin Burniston
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 23:21:30	ID requested
1	2023-06-27 04:11:47	announced
⏵ 2	2023-11-14 09:01:48	announced	2023-11-14: Updated project metadata.

Nishimura Y, Bittel AJ, Stead CA, Chen YW, Burniston JG, Facioscapulohumeral Muscular Dystrophy is Associated With Altered Myoblast Proteome Dynamics. Mol Cell Proteomics, 22(8):100605(2023) [pubmed]

submitter keyword: Human, FUSION

Jatin G Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
lab head
Jatin Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038818
     1. Label: PRIDE project
     2. Name: D2O proteome dynamics in FSHD vs UASb myoblasts