PXD038813

PXD038813 is an original dataset announced via ProteomeXchange.

Title	Efficient sugar utilization and transition from oxidative to substrate-level phosphorylation in high starch storage roots of African cassava genotypes
Description	Cassava’s storage roots represent one of the most important sources of nutritional carbohydrates worldwide. Particularly, smallholder farmers in Sub-Saharan Africa depend on this crop plant, where resilient and yield-improved varieties are of vital importance to support steadily increasing populations. Aided by a growing understanding of the plant’s metabolism and physiology, targeted improvement concepts already led to visible gains in recent years. To expand our knowledge and to contribute to these successes, we investigated storage roots of eight cassava genotypes with differential dry matter content from three successive field trials for their proteomic and metabolic profiles. At large, the metabolic focus in storage roots transitioned from cellular growth processes towards carbohydrate and nitrogen storage with increasing dry matter content. This is reflected in higher abundance of proteins related to nucleotide synthesis, protein turnover and vacuolar energization in low starch genotypes, while proteins involved in sugar conversion and glycolysis were more prevalent in high dry matter genotypes. This shift in metabolic orientation was underlined by a clear transition from oxidative- to substrate-level phosphorylation in high dry matter genotypes. Our analyses highlight metabolic patterns that are consistently and quantitatively associated with high dry matter accumulation in cassava storage roots, providing fundamental understandings of cassava’s metabolism as well as a data resource for targeted genetic improvement.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:49:27.092.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038813
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Lamm
SpeciesList	scientific name: Manihot esculenta (Cassava) (Jatropha manihot); NCBI TaxID: 3983;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 22:58:42	ID requested
1	2023-06-21 04:45:32	announced
2	2023-11-14 06:45:26	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:49:27	announced	2024-10-22: Updated project metadata.

10.6019/PXD038813;

10.1111/TPJ.16357;

submitter keyword: cassava

Uwe Sonnewald
contact affiliation	Friedrich-Alexander-Universität Erlangen-Nürnberg, Division of Biochemistry, Erlangen, Germany
contact email	Uwe.Sonnewald@fau.de
lab head
Christian Lamm
contact affiliation	Friedrich-Alexander Universität Erlangen-Nürnberg
contact email	Christian.lamm@fau.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038813
     1. Label: PRIDE project
     2. Name: Efficient sugar utilization and transition from oxidative to substrate-level phosphorylation in high starch storage roots of African cassava genotypes