PXD038803

PXD038803 is an original dataset announced via ProteomeXchange.

Title	Metrologically traceable quantification of three apolipoprotein E isoforms in cerebrospinal fluid
Description	Apolipoprotein E (apoE) is a 317-amino-acid long glycoprotein with three common isoforms (apoE2, apoE3, and apoE4) encoded by three apoE alleles (ε2, ε3, and ε4). These polymorphic forms arise from single amino acid substitutions with Cys and Arg interchanged at positions 112 and 158 (mature protein)1. Measuring the cerebrospinal fluid (CSF) concentrations of total apoE and the specific isoforms might provide new insights to neurological processes and neuropathogenesis. This method employs a bottom-up approach to quantify each individual apoE isoform concentration and the total concentration of apoE in CSF. Following sequential denaturation, reduction, and alkylation, the proteins are enzymatically cleaved with trypsin to liberate peptides. The resulting CSF digests are then injected into an LC-MS/MS system. Five surrogate apoE peptides are monitored. The peptides CLAVYQAGAR (abbreviated as CLAVY) and LGADMEDVR (abbreviated LGAD) were selected as specific peptide for apoE2 and E4, respectively. The peptide LGADMEDVCGR (abbreviated as LGAD-C) was selected as a common peptide for apoE2 and apoE3, while the peptide LAVYQAGAR (abbreviated as LAVY) was used as a common peptide for apoE3 and apoE4. The peptide SELEEQLTPVAEETR (abbreviated as SELEE) was selected as a common peptide of all three isoforms.
HostingRepository	PanoramaPublic
AnnounceDate	2023-06-13
AnnouncementXML	Submission_2023-06-13_10:47:22.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Huu Hien Huynh
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(4)
Instrument	Xevo TQ-S

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 15:44:48	ID requested
⏵ 1	2023-06-13 10:47:23	announced

Huynh HH, Kuch K, Orquillas A, Forrest K, Barahona-Carrillo L, Keene D, Henderson VW, Wagner AD, Poston KL, Montine TJ, Lin A, Tian L, MacCoss MJ, Emrick MA, Hoofnagle AN, Metrologically Traceable Quantification of 3 Apolipoprotein E Isoforms in Cerebrospinal Fluid. Clin Chem, 69(7):734-745(2023) [pubmed]

submitter keyword: apolipoprotein E, isoform, Alzheimer’s disease, single-point calibration, neurodegenerative disorders

Andrew N. Hoofnagle
contact affiliation	University of Washington
contact email	ahoof@uw.edu
lab head
Huu Hien Huynh
contact affiliation	University of Washington
contact email	hhuynh89@uw.edu
dataset submitter

Panorama Public dataset URI