PXD038785 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy |
Description | Purified, putative unprocessed and processed MBPmut-TNFa fusion proteins were analyzed by LC-MS. The average molecular weights of the eluted MBPmut-TNFa fusion proteins obtained by deconvolution of the ESI-MS spectra are given. For MBPmut-TNFa preparations from “Empty vector control” and “RseP-Myc H22F”, we found a protein eluting at 9.1 with an average molecular weight of about 45448 Da min, which was higher than expected for a MBPmut-TNFa fusion protein and was therefore not further characterized. Compounds eluting at retention times above 9.5 min (corresponding to 90 % acetonitrile) were contaminants not further characterized. a to e belong to one experiment, and the processing specificity of RseP wt was confirmed in this experiment by analysis of RsePMyc wt. The C-terminal sequences of the substrate MBPmut- … CPFLSLFSFL39 fusion protein and processing product MBPmut- … CPFLSLF36 were confirmed by LC-MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:44:22.102.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD038785 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rabea Goetz |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | impact II |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-12 23:05:47 | ID requested | |
1 | 2023-03-20 06:04:25 | announced | |
⏵ 2 | 2023-11-14 08:44:30 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD038785; |
Kupke T, G, ö, tz RM, Richter FM, Beck R, Lolicato F, Nickel W, Hopf C, Br, ü, gger B, In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy. Commun Biol, 6(1):287(2023) [pubmed] |
Keyword List
submitter keyword: TNF, fusion protein, intact mass analysis,LC-MSMS, intermembrane protease |
Contact List
Carsten Hopf |
contact affiliation | Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany |
contact email | c.hopf@hs-mannheim.de |
lab head | |
Rabea Goetz |
contact affiliation | Mannheim University of Applied Sciences, Center for Mass Spectrometry and Optical Spectroscopy |
contact email | r.goetz@hs-mannheim.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038785
- Label: PRIDE project
- Name: In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy