PXD038785

PXD038785 is an original dataset announced via ProteomeXchange.

Title	In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy
Description	Purified, putative unprocessed and processed MBPmut-TNFa fusion proteins were analyzed by LC-MS. The average molecular weights of the eluted MBPmut-TNFa fusion proteins obtained by deconvolution of the ESI-MS spectra are given. For MBPmut-TNFa preparations from “Empty vector control” and “RseP-Myc H22F”, we found a protein eluting at 9.1 with an average molecular weight of about 45448 Da min, which was higher than expected for a MBPmut-TNFa fusion protein and was therefore not further characterized. Compounds eluting at retention times above 9.5 min (corresponding to 90 % acetonitrile) were contaminants not further characterized. a to e belong to one experiment, and the processing specificity of RseP wt was confirmed in this experiment by analysis of RsePMyc wt. The C-terminal sequences of the substrate MBPmut- … CPFLSLFSFL39 fusion protein and processing product MBPmut- … CPFLSLF36 were confirmed by LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:22.102.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038785
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rabea Goetz
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 23:05:47	ID requested
1	2023-03-20 06:04:25	announced
⏵ 2	2023-11-14 08:44:30	announced	2023-11-14: Updated project metadata.

10.6019/PXD038785;

Kupke T, G, ö, tz RM, Richter FM, Beck R, Lolicato F, Nickel W, Hopf C, Br, ü, gger B, In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy. Commun Biol, 6(1):287(2023) [pubmed]

submitter keyword: TNF, fusion protein, intact mass analysis,LC-MSMS, intermembrane protease

Carsten Hopf
contact affiliation	Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany
contact email	c.hopf@hs-mannheim.de
lab head
Rabea Goetz
contact affiliation	Mannheim University of Applied Sciences, Center for Mass Spectrometry and Optical Spectroscopy
contact email	r.goetz@hs-mannheim.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038785

PRIDE project URI

• PRIDE
  1. PXD038785
     1. Label: PRIDE project
     2. Name: In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy