PXD038765

PXD038765 is an original dataset announced via ProteomeXchange.

Title	Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients
Description	Serum autoantibodies targeting the SSA/Ro proteins are a key component of the classification criteria for the diagnosis of Sjögren’s syndrome (SS). Most patient serum reacts with both Ro60 and Ro52 proteins. Here we compare the molecular and clinical characteristics of patients diagnosed with SS with anti-Ro52 in the presence or absence of anti-Ro60/La autoantibodies. Patients in the SS biobank at Westmead Hospital that were positive for anti-Ro52 were included and stratified based on the absence (isolated) or presence (combined) of anti-Ro60/La, measured by line immunoassay. We examined clinical associations and the serological and molecular characteristics of anti-Ro52 using ELISA and mass spectrometry in serological groups. A total of 123 SS patients were included for study. SS patients with isolated anti-Ro52 (12%) identified a severe serological subset characterised by higher disease activity, vasculitis, pulmonary involvement, rheumatoid factor (RhF) and cryoglobulinaemia. Anti-Ro52 IgG titre was correlated with RhF. Serum antibodies reacting with Ro52 in the isolated anti-Ro52 subset displayed less isotype switching, less immunoglobulin variable region subfamily usage and a lower degree of somatic hypermutation than the combined anti-Ro52 subset. In our cohort of SS patients, isolated anti-Ro52 represents a severe subset of SS, and is independently associated with the presence of cryoglobulinaemia. We therefore provide clinical relevance to the stratification of SS patients by their sero-reactivities. It is possible that the autoantibody patterns may be immunological epiphenomena of the underlying disease process, and further work is required to unearth the mechanisms of the differential clinical phenotypes.
HostingRepository	PRIDE
AnnounceDate	2023-03-15
AnnouncementXML	Submission_2023-03-14_17:48:52.620.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038765
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jing JingWang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 22:44:45	ID requested
⏵ 1	2023-03-14 17:48:52	announced

10.6019/PXD038765;

submitter keyword: anti-Ro52/TRIM21

autoantibodies

cryoglobulinaemia

rheumatoid factor

Sjögren’s syndrome

Jing JingWang
contact affiliation	Immunology,College of Medicine and Public Health, Flinders University
contact email	jingjing.wang@flinders.edu.au
lab head
Jing JingWang
contact affiliation	Flinders University
contact email	jingjing.wang@flinders.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038765
     1. Label: PRIDE project
     2. Name: Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients