PXD038765 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients |
Description | Serum autoantibodies targeting the SSA/Ro proteins are a key component of the classification criteria for the diagnosis of Sjögren’s syndrome (SS). Most patient serum reacts with both Ro60 and Ro52 proteins. Here we compare the molecular and clinical characteristics of patients diagnosed with SS with anti-Ro52 in the presence or absence of anti-Ro60/La autoantibodies. Patients in the SS biobank at Westmead Hospital that were positive for anti-Ro52 were included and stratified based on the absence (isolated) or presence (combined) of anti-Ro60/La, measured by line immunoassay. We examined clinical associations and the serological and molecular characteristics of anti-Ro52 using ELISA and mass spectrometry in serological groups. A total of 123 SS patients were included for study. SS patients with isolated anti-Ro52 (12%) identified a severe serological subset characterised by higher disease activity, vasculitis, pulmonary involvement, rheumatoid factor (RhF) and cryoglobulinaemia. Anti-Ro52 IgG titre was correlated with RhF. Serum antibodies reacting with Ro52 in the isolated anti-Ro52 subset displayed less isotype switching, less immunoglobulin variable region subfamily usage and a lower degree of somatic hypermutation than the combined anti-Ro52 subset. In our cohort of SS patients, isolated anti-Ro52 represents a severe subset of SS, and is independently associated with the presence of cryoglobulinaemia. We therefore provide clinical relevance to the stratification of SS patients by their sero-reactivities. It is possible that the autoantibody patterns may be immunological epiphenomena of the underlying disease process, and further work is required to unearth the mechanisms of the differential clinical phenotypes. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-15 |
AnnouncementXML | Submission_2023-03-14_17:48:52.620.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD038765 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jing JingWang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-12 22:44:45 | ID requested | |
⏵ 1 | 2023-03-14 17:48:52 | announced | |
Publication List
Keyword List
submitter keyword: anti-Ro52/TRIM21 |
autoantibodies |
cryoglobulinaemia |
rheumatoid factor |
Sjögren’s syndrome |
Contact List
Jing JingWang |
contact affiliation | Immunology,College of Medicine and Public Health, Flinders University |
contact email | jingjing.wang@flinders.edu.au |
lab head | |
Jing JingWang |
contact affiliation | Flinders University |
contact email | jingjing.wang@flinders.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038765
- Label: PRIDE project
- Name: Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients