PXD038748

PXD038748 is an original dataset announced via ProteomeXchange.

Title	COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy
Description	Severe neurological symptoms are associated with Coronavirus disease 2019 (COVID-19). However, the morphologic features, pathological nature and their potential mechanisms in patient brains have not been revealed despite evidence of neurotropic infection. In this study, neuropathological damages and infiltrating inflammatory cells were quantitatively evaluated by immunohistochemical staining, ultrastructural examination under electron microscopy, and a pixel threshold method, in postmortem brains from nine critically ill COVID-19 patients and nine age-matched cadavers of healthy individuals. Differentially expressed proteins were identified by quantitative proteomic assays. Histopathological findings included neurophagocytosis, microglia nodules, satellite phenomena, extensive edema, focal hemorrhage, and infarction, as well as infiltrating mononuclear cells. Immunostaining of COVID-19 brains revealed extensive activation of both microglia and astrocytes, severe damage of blood-brain barrier (BBB) and various degrees of perivascular infiltration by predominantly CD14+/CD16+/CD141+/CCR7+/CD11c+ monocytes and occasionally CD4+/CD8+ T lymphocytes in. Quantitative proteomic assays combined with bioinformatics analysis identified upregulated proteins predominantly involved in immune responses, autophagy and cellular metabolism in COVID-19 patient brains compared with control brains. Proteins involved in brain development, neuroprotection, and extracellular matrix proteins of basement membrane were downregulated, potentially caused by the activation of transforming growth factor β receptor and vascular endothelial growth factor signaling pathways. Thus, our results define histopathological and molecular profiles of COVID-19-associated monocytic encephalitis (CAME) and suggest potential therapeutic targets.
HostingRepository	iProX
AnnounceDate	2022-12-12
AnnouncementXML	Submission_2024-03-20_00:14:40.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peipei Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 05:24:43	ID requested
⏵ 1	2024-03-20 00:14:41	announced

Zhang PP, He ZC, Yao XH, Tang R, Ma J, Luo T, Zhu C, Li TR, Liu X, Zhang D, Zhang S, Ping YF, Leng L, Bian XW, COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy. Signal Transduct Target Ther, 8(1):24(2023) [pubmed]

submitter keyword: Encephalitis, COVID-19, Autopsy, Proteomics

Xiuwu Bian
contact affiliation	Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing 400038, China
contact email	bianxiuwu@263.net
lab head
Peipei Zhang
contact affiliation	Department of Pathology, the First Affiliated Hospital of University of Science & Technology of China, Hefei, Anhui 230036, China.
contact email	zhangpeipei_sh@163.com
dataset submitter

iProX dataset URI