PXD038747 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The mass spectrometry-based proteomics analysis of macrophages |
Description | Defective autophagy is linked to proinflammatory diseases. However, the mechanisms by which autophagy limits inflammation remain elusive. Here, we found that the pan-FGFR inhibitor LY2874455 efficiently activated autophagy and suppressed innate inflammation in macrophages stimulated by lipopolysaccharide (LPS). The mass spectrometry-based proteomics analysis of RAW264.7 cells treated with LPS (20ng/ml) and LY2874455 was performed. The protein levels of macrophages were systematically analyzed and quantified. Then, the protein levels were compared. The fold change and significance were analyzed. Multiplex proteomic profiling identified the immunoproteasome, a specific isoform of the 20s constitutive proteasome, as substrates that are degraded by selective autophagy. SQSTM1/p62 was found to be a selective autophagy-related receptor that mediated this degradation. Autophagy deficiency or p62 knockdown blocked the effects of LY2874455, leading to the accumulation of immunoproteasomes and increases in inflammatory reactions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:42:06.456.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kefeng Lu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-12 04:58:25 | ID requested | |
1 | 2024-05-24 00:57:14 | announced | |
⏵ 2 | 2024-10-22 06:42:10 | announced | 2024-10-22: Updated project metadata. |
Publication List
Zhou J, Li C, Lu M, Jiang G, Chen S, Li H, Lu K, Pharmacological induction of autophagy reduces inflammation in macrophages by degrading immunoproteasome subunits. PLoS Biol, 22(3):e3002537(2024) [pubmed] |
10.1371/journal.pbio.3002537; |
Keyword List
submitter keyword: Macrophage |
LPS |
immunoproteasome |
Contact List
Kefeng Lu |
contact affiliation | Sichuan University |
contact email | lukf@scu.edu.cn |
lab head | |
Kefeng Lu |
contact affiliation | Sichuan University |
contact email | lukf@scu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038747
- Label: PRIDE project
- Name: The mass spectrometry-based proteomics analysis of macrophages