PXD038745

PXD038745 is an original dataset announced via ProteomeXchange.

Title	DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice.
Description	Among numerous enzymes involved in RNA decay, processive exoribonucleases are the most prominent group responsible for the degradation of the entire RNA molecules. The role of mammalian cytoplasmic 3’-5’ exonuclease DIS3L at the organismal level remained unknown. Herein we established knock-in and knock-out mouse models to study DIS3l functions in mice. DIS3L is indeed a subunit of the cytoplasmic exosome complex, which disruption leads to severe embryo degeneration and death in mice soon after implantation. These changes could not be prevented by supplementing extraembryonic tissue with functional DIS3L through the construction of chimeric embryos. Preimplantation DIS3L KO embryos were unaffected in their morphology and ability to produce functional embryonic stem cells (ES) cells showing that DIS3L is not essential for cell viability. There were also no major changes in the transcriptome level for both ES cells and blastocysts, as revealed by RNA sequencing experiments. Notably, however, DIS3l KO led to inhibition of the global protein synthesis. These results point to the essential role of DIS3l in mRNA quality control pathways crucial for proper protein synthesis during embryo development.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:12:03.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Cysewski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 04:57:46	ID requested
⏵ 1	2025-05-06 09:12:04	announced

10.1261/rna.080350.124;

Brouze M, Szpila M, Czerwi, ń, ska A, Antczak W, Mroczek S, Kuli, ń, ski TM, Hojka-Osi, ń, ska A, Cysewski D, Gewartowska O, Adamska D, Gruchota J, Borsuk E, Dziembowski A, DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice. RNA, 31(5):646-662(2025) [pubmed]

submitter keyword: DIS3L, RNA

Andrzej Dziembowski
contact affiliation	Laboratory of RNA Biology International Institute of Molecular and Cell Biology in Warsaw
contact email	adziembowski@iimcb.gov.pl
lab head
Dominik Cysewski
contact affiliation	Medical University of Bialystok
contact email	dominikcysewski@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038745
     1. Label: PRIDE project
     2. Name: DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice.