PXD038742

PXD038742 is an original dataset announced via ProteomeXchange.

Title	A deeply cA deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis
Description	Reactive oxygen species (ROS) are constant by-products of aerobic life. In excess, ROS lead to cytotoxic protein aggregates, which are a hallmark of ageing in animals and linked to age-related pathologies in humans. Acylamino acid-releasing enzymes (AARE) are bifunctional serine proteases, acting on oxidized proteins. AARE are found in all domains of life, albeit under different names, such as acylpeptide hydrolase (APEH/ACPH), acylaminoacyl peptidase (AAP), or oxidized protein hydrolase (OPH). In humans, AARE malfunction is associated with age-related pathologies, while their function in plants is less clear. Here, we provide a detailed analysis of AARE genes in the plant lineage and an in-depth analysis of AARE localization and function in the moss Physcomitrella and the angiosperm Arabidopsis. AARE loss-of-function mutants have not been described for any organism so far. We generated and analysed such mutants and describe a connection between AARE function, aggregation of oxidized proteins and plant ageing, including accelerated developmental progression and reduced life span. Our findings complement similar findings in animals and humans, and suggest a unified concept of ageing may exist in different life forms
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:32.957.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038742
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ralf Reski
SpeciesList	scientific name: Physcomitrella; NCBI TaxID: 3217;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 04:56:20	ID requested
1	2023-03-11 10:32:25	announced
⏵ 2	2023-11-14 08:43:38	announced	2023-11-14: Updated project metadata.

Hoernstein SNW, Ö, zdemir B, van Gessel N, Miniera AA, Rogalla von Bieberstein B, Nilges L, Schweikert Farinha J, Komoll R, Glauz S, Weckerle T, Scherzinger F, Rodriguez-Franco M, M, ü, ller-Sch, ü, ssele SJ, Reski R, A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis. Commun Biol, 6(1):61(2023) [pubmed]

10.6019/PXD038742;

submitter keyword: plant ageing, ROS,aging, Physcomitrium, protein oxidation

Ralf Reski
contact affiliation	Head, Chair Plant Biotechnology Faculty of Biology University of Freiburg Schaenzlestrasse 1 D-79104 Freiburg Germany
contact email	ralf.reski@biologie.uni-freiburg.de
lab head
Ralf Reski
contact affiliation	Faculty of Biology, University of Freiburg (Chair Plant Biotechnology), Schaenzlestr. 1, D-79104 Freiburg
contact email	ralf.reski@biologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038742
     1. Label: PRIDE project
     2. Name: A deeply cA deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis