PXD038740

PXD038740 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of side and non-side populations isolated from A549 lung carcinoma cells cultivated under normoxic and hypoxic conditions
Description	Presence of key hypoxic regulators, HIF-1α or HIF-2α, in tumors is associated with disease progression and poor patient prognosis. Hypoxia massively activates several genes including the one encoding BCRP transport protein that proffers the multidrug resistance to cancer cells through the xenobiotic efflux and, moreover, is a determinant of side population (SP) that associates with cancer stem-like phenotype. As the natural medicine comes to the fore, it is instinctive to look for natural agents possessing powerful features against cancer resistance. Hypericin, pleiotropic agent found in Hypericum plants, is good example, as it is BCRP substrate and potential inhibitor, as well as SP- and HIF-modulator. Here, we showed that hypericin efficiently accumulates in hypoxic cancer cells, degrades HIF-1/2α and together with hypoxia decreases BCRP efflux, thus diminishes SP population. On the contrary, this seemingly favorable result was accompanied by stimulated migration of this minor population of cancer cells that preserved SP phenotype. Since hypoxia unexpectedly decreased BCRP level and SP fraction, we also compared the SP and nonSP proteomes and their changes under hypoxia in A549 cell line. We identified differences especially among protein groups connected to the epithelial-mesenchymal transition, although major changes were related to hypoxia, as upregulation of many proteins, including serpin E1, PLOD2 and LOXL2, that ultimately contribute to the initiation of the metastatic cascade, were detected. Altogether, this study represents another piece of puzzle that clarify the innate and hypoxia-triggered resistance of cancer cells and highlights the ambivalent role of natural agents in biology of these cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:06.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 04:52:58	ID requested
1	2023-05-03 23:24:16	announced
⏵ 2	2023-11-14 09:00:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Breast cancer resistance protein, Side population, Q-Exactive,Hypericin, Proteomics, ECM reorganization, Hypoxia

Zbyněk Zdráhal
contact affiliation	National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD038740

PRIDE project URI

• PRIDE
  1. PXD038740
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of side and non-side populations isolated from A549 lung carcinoma cells cultivated under normoxic and hypoxic conditions