PXD038734

PXD038734 is an original dataset announced via ProteomeXchange.

Title	Detect the shared protein components between spider dragline silk and silkworm cocoon by Label free quantitative proteomics
Description	The spider major ampullate gland and silkworm silk gland have similar morphological structure and function, so we explored whether there are some common protein components on silks, with three biological replicates for each sample. Dragline silk samples from mature female Trichonephila clavata spider and cocoon samples are from silkworm Bombyx mori chain. The dragline silk of the spider T. clavata and cocoon silk of the silkworm B. mori were cut with scissors. First, silk samples (20 mg of each sample) were dissolved in 500 μL of 9 M LiSCN, and the supernatant was collected after centrifugation (10,000 × g, 10 min). Protein concentrations were measured via the Bradford protein assay (Beyotime, China). Then, the samples were diluted 10-fold in an 8 M urea solution, mixed with 5 × SDS–PAGE buffer, and separated using a NuPAGE 4–12% Bis-Tris protein gel (Thermo Fisher Scientific, USA). The samples were subsequently digested using trypsin, and peptides were measured via LC–MS/MS on a Q Exactive HF-X column.
HostingRepository	iProX
AnnounceDate	2022-12-09
AnnouncementXML	Submission_2023-02-20_19:00:31.942.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anqiang Jia
SpeciesList	scientific name: Bombyx mori; NCBI TaxID: 7091; scientific name: Trichonephila; NCBI TaxID: 2585208;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 00:12:34	ID requested
1	2022-12-12 00:12:45	announced
⏵ 2	2023-02-20 19:00:37	announced	2023-02-21: Update information.

Hu W, Jia A, Ma S, Zhang G, Wei Z, Lu F, Luo Y, Zhang Z, Sun J, Yang T, Xia T, Li Q, Yao T, Zheng J, Jiang Z, Xu Z, Xia Q, Wang Y, A molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk. Nat Commun, 14(1):837(2023) [pubmed]

submitter keyword: Dragline silk, silkworm cocoon, LC-MS

Yi Wang
contact affiliation	Southwest Univeristy, Biological Research Center
contact email	yiwang28@swu.edu.cn
lab head
Anqiang Jia
contact affiliation	Southwest Univeristy, Biological Research Center
contact email	jiaanqiang@email.swu.edu.cn
dataset submitter

iProX dataset URI