PXD038716

PXD038716 is an original dataset announced via ProteomeXchange.

Title	Quantitative mass spectrometry analysis of 70S ribosomes from iSAT
Description	The ribosome is one of the largest and the most complicated enzymes in cells, making up 25% of the bacterial cell’s dry mass, and is responsible for protein synthesis in all organisms on earth. In 2013, Jewett et al. reported an in vitro system named integrated rRNA synthesis, ribosome assembly, and translation (iSAT) reaction, which provided a defined system for ribosome biogenesis in a near-physiological environment.In this project, the r-protein composition in 30S and 50S ribosomeal subunits assembled in the iSAT reaction was quantified by mass spectrometry compared to 15N labeled 70S ribosomes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:03.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiyu Dong
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-09 05:25:01	ID requested
1	2023-05-10 13:48:37	announced
⏵ 2	2023-11-14 08:47:04	announced	2023-11-14: Updated project metadata.

Dong X, Doerfel LK, Sheng K, Rabuck-Gibbons JN, Popova AM, Lyumkis D, Williamson JR, Near-physiological in vitro assembly of 50S ribosomes involves parallel pathways. Nucleic Acids Res, 51(6):2862-2876(2023) [pubmed]

submitter keyword: in vitro, 70S,ribosome, LC-MS/MS

James R. Williamson
contact affiliation	Department of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
contact email	jrwill@scripps.edu
lab head
Xiyu Dong
contact affiliation	Scripps Research Institute
contact email	xdong@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD038716

PRIDE project URI

• PRIDE
  1. PXD038716
     1. Label: PRIDE project
     2. Name: Quantitative mass spectrometry analysis of 70S ribosomes from iSAT