PXD038714

PXD038714 is an original dataset announced via ProteomeXchange.

Title	The Thylakoid Protein NTA1 Is An Assembly Factor of Cytochrome b6f Complex Essential for Chloroplast Development in Arabidopsis
Description	The cytochrome b6f (Cyt b6f) complex is a multisubunit protein complex in chloroplast thylakoid membranes required for photosynthetic electron transport. Here we report the isolation and characterization of the new tiny albino (nta1) mutant in Arabidopsis, which had severe defects in Cyt b6f accumulation and chloroplast development. Gene cloning unveiled that the nta1 phenotypes were caused by the disruption of a single nuclear gene, NTA1, which encodes an integral thylakoid membrane protein conserved across green algae and plants. Overexpression of NTA1 completely rescued the nta1 phenotypes, while knockout of NTA1 in wild type plants recapitulated the mutant phenotypes. Loss of NTA1 function severely impaired the accumulation of multiprotein complexes related to photosynthesis in thylakoid membranes, particularly the components of Cyt b6f. Furthermore, NTA1 was shown to directly interact with four subunits (Cyt b6/PetB, PetD, PetG and PetN) of Cyt b6f through the DUF1279 domain and C-terminal sequence to mediate their assembly. Taken together, our study identified NTA1 as a new and key regulator of chloroplast development, which plays essential roles in the assembly of Cyt b6f complex through interacting with multiple Cyt b6f subunits.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:36.396.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Feng
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-09 03:31:02	ID requested
1	2023-02-22 01:09:33	announced
⏵ 2	2023-11-14 08:58:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Albino mutant, Assembly factor, Cytochrome b6f complex, Chloroplast development

Lei Feng
contact affiliation	The Chinese University of Hong Kong
contact email	lfeng@link.cuhk.edu.hk
lab head
Lei Feng
contact affiliation	The Chinese University of Hong Kong
contact email	lfeng@link.cuhk.edu.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038714
     1. Label: PRIDE project
     2. Name: The Thylakoid Protein NTA1 Is An Assembly Factor of Cytochrome b6f Complex Essential for Chloroplast Development in Arabidopsis