PXD038710

PXD038710 is an original dataset announced via ProteomeXchange.

Title	Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacterium Hydrogenophaga sp. H7
Description	Nitrogen and arsenic contaminants often coexist in groundwater, and microbes show the potential for simultaneous removal of nitrogen and arsenic. Here, we reported that Hydrogenophaga sp. H7 was heterotrophic nitrification and aerobic denitrification (HNAD) and arsenite [As(III)] oxidation bacterium. Strain H7 presented efficient capacities for simultaneous NH4+-N, NO3--N, or NO2--N removal with As(III) oxidation during aerobic cultivation. Strikingly, the bacterial ability to remove nitrogen and oxidize As(III) has remained high across a wide range of temperatures, pH values, and shaking speeds, exceeding that of the most commonly reported HNAD bacteria. Additionally, the previous HNAD strains exhibited a high denitrification efficiency, but a suboptimal concentration of nitrogen remained in the wastewater. Here, strain H7 combined with FeCl3 efficiently removed 96.14% of NH4+-N, 99.08% of NO3--N, and 94.68% of total nitrogen (TN), and it oxidized 100% of As(III), even at a low nitrogen concentration (35 mg/L). The residues in the wastewater still met the Surface Water Environmental Quality Standard of China after five continuous wastewater treatment cycles. Furthermore, genome and proteomic analyses led us to propose that the shortcut nitrification-denitrification pathway and As(III) oxidase AioBA are the key pathways that participate in simultaneous nitrogen removal and As(III) oxidation.
HostingRepository	iProX
AnnounceDate	2022-12-09
AnnouncementXML	Submission_2023-03-28_20:31:34.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaixiang Shi
SpeciesList	scientific name: Hydrogenophaga sp. H7; NCBI TaxID: 1882399;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-09 02:12:48	ID requested
1	2022-12-09 02:13:02	announced
⏵ 2	2023-03-28 20:31:36	announced	2023-03-29: Update publication information.

Fan X, Nie L, Chen Z, Zheng Y, Wang G, Shi K, sp. H7. Front Microbiol, 13():1103913(2022) [pubmed]

submitter keyword: Hydrogenophaga, nitrogen removal, arsenitae oxidation, wastewater microbial treatment, cocontamination

Kaixiang Shi
contact affiliation	Huazhong Agriculture University
contact email	kaixiangshi@mail.hzau.edu.cn
lab head
Kaixiang Shi
contact affiliation	Huazhong Agriculture University
contact email	kaixiangshi@mail.hzau.edu.cn
dataset submitter

iProX dataset URI