PXD038706

PXD038706 is an original dataset announced via ProteomeXchange.

Title	Comparison of the photoprotection mechanism in Chlorella Ohadii and Chlamydomonas reinhardtii
Description	Light is the driving force of photosynthesis. However, excessive light can be harmful. Photoinhibition, or lightinduced photodamage, is one of the key processes limiting photosynthesis. When the absorbed light exceeds the amount that can be dissipated by photosynthetic electron flow and other processes, damaging radicals are formed that mostly inactivate photosystem II (PSII). A well defined mechanism that protects the photosynthetic apparatus from photoinhibition has been described in the model green alga Chlamydomonas reinhardtii and plants. Chlorella ohadii is a green microalga, isolated from biological desert soil crusts, that thrives under extreme high light (HL) in which other organisms do not survive. Here, we show that this alga evolved unique protection mechanisms distinct from those of C. reinhardtii and plants. When grown under extreme HL, significant structural changes were noted in the C. ohadii thylakoids, including a drastic reduction in the antennae and the formation of stripped core PSII, lacking its outer and inner antennae. This is accompanied by a massive accumulation of protective carotenoids and proteins that scavenge harmful radicals. At the same time, several elements central to photoinhibition protection in C. reinhardtii, such as psbS, the stress related light harvesting complex (LHCSR), PSII protein phosphorylation and state transitions are entirely absent or were barely detected in C. ohadii.
HostingRepository	PRIDE
AnnounceDate	2023-05-08
AnnouncementXML	Submission_2023-05-08_10:58:52.358.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	OdedKleifeld
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; scientific name: Chlorella ohadii; NCBI TaxID: 2649997;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-08 16:48:44	ID requested
⏵ 1	2023-05-08 10:58:52	announced

submitter keyword: photosynthesis,Chlorella Ohadii, phosphorylation, Chlamydomonas reinhardtii

GadiSchuster
contact affiliation	Faculty of Biology Technion Haifa 32000 Israel
contact email	gadis@technion.ac.il
lab head
OdedKleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD038706

PRIDE project URI

• PRIDE
  1. PXD038706
     1. Label: PRIDE project
     2. Name: Comparison of the photoprotection mechanism in Chlorella Ohadii and Chlamydomonas reinhardtii