PXD038703

PXD038703 is an original dataset announced via ProteomeXchange.

Title	Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses
Description	Fast synaptic inhibition is dependent on targeting specific GABAAR subtypes to dendritic and axon initial segment (AIS) synapses. Synaptic GABAARs are typically assembled from 1-3,  and subunits. Here, we isolate distinct GABAARs from the brain and interrogate their composition using quantitative proteomics. We show that α2-containing receptors co-assemble with α1 subunits, whereas α1 receptors can form GABAARs with α1 as the sole  subunit. We demonstrate that α1 and α2 subunit-containing receptors co-purify with distinct spectrin isoforms; cytoskeletal proteins that link transmembrane proteins to the cytoskeleton. β2-spectrin was preferentially associated with α1-containing GABAARs at dendritic synapses, while β4-spectrin was associated with 2-containing GABAARs at AIS synapses. Ablating β2-spectrin expression reduced dendritic and AIS synapses containing α1 but increased the number of synapses containing α2, which altered phasic inhibition. Thus, we demonstrate a role for spectrins in the synapse-specific targeting of GABAARs, determining the efficacy of fast neuronal inhibition.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:26:20.624.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038703
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joshua Smalley
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-08 16:45:29	ID requested
1	2023-03-10 13:53:09	announced
⏵ 2	2023-11-14 08:26:21	announced	2023-11-14: Updated project metadata.

10.6019/PXD038703;

Smalley JL, Cho N, Ng SFJ, Choi C, Lemons AHS, Chaudry S, Bope CE, Dengler JS, Zhang C, Rasband MN, Davies PA, Moss SJ, receptors at somatodendritic synapses. Commun Biol, 6(1):11(2023) [pubmed]

submitter keyword: AIS,GABA A Receptors, Spectrins

Stephen J Moss
contact affiliation	Department of Neuroscience , Tufts University
contact email	stephen.moss@tufts.edu
lab head
Joshua Smalley
contact affiliation	Tufts University
contact email	joshua.smalley@tufts.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038703

PRIDE project URI

• PRIDE
  1. PXD038703
     1. Label: PRIDE project
     2. Name: Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses