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PXD038685

PXD038685 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer.
DescriptionIxodes scapularis and other hard ticks are long-term blood feeders. This feeding behavior is facilitated by a tick secreted bio adhesive (tick cement) that attaches tick mouthparts to skin tissue and prevents the host from dislodging the tick from its feeding site. Understanding tick cement formation is highly sought after as its disruption will prevent tick feeding. This study describes proteins that form the inner core layer of I. scapularis tick cement as disrupting these will stop formation of the outer cortical layer. As the inner core cement layer completes forming by 24 h of attachment, we used laser capture to isolate cement from cryosections of 6 h and 24 h tick attachment sites and to distinguish between early and late inner core cement proteins. LC-MS/MS analysis identified 138 tick cement proteins (TCPs) of which 37 and 35 were unique in cement of 6 and 24 h respectively, and 66 shared proteins. The 138 TCPs are classified in 14 functional categories: cuticular protein (16%), tick specific proteins of unknown function, cytoskeletal proteins, and enzymes (at 13% each), enzymes (10%), antioxidant, glycine rich, scaffolding, heat shock, histone, histamine binding, proteases and protease inhibitors, and miscellaneous (at 3-6% each). Gene ontology analysis confirm that TCPs are enriched for bio adhesive properties. Our data offer insights into yet unknown tick cement bonding patterns and set the foundation for understanding the molecular basis of I. scapularis tick cement formation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:43:58.739.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD038685
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlbert Mulenga
SpeciesList scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-08 13:43:39ID requested
12023-03-11 11:47:21announced
22023-11-14 08:44:03announced2023-11-14: Updated project metadata.
Publication List
Mulenga A, Radulovic Z, Porter L, Britten TH, Kim TK, Tirloni L, Gaithuma AK, Adeniyi-Ipadeola GO, Dietrich JK, Moresco JJ, Yates JR, Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer. Sci Rep, 12(1):21300(2022) [pubmed]
10.6019/PXD038685;
Keyword List
submitter keyword: Ixodes scapularis tick cement, Laser capture micro dissection, LC-MS/MS
Contact List
Albert Mulenga
contact affiliationDepartment of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas,United States of America
contact emailamulenga@cvm.tamu.edu
lab head
Albert Mulenga
contact affiliationTexas A&M University
contact emailmulengalab06@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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