PXD038669

PXD038669 is an original dataset announced via ProteomeXchange.

Title	Method development for simplified High-Throughput Plasma Proteomics
Description	We developed a simplified proteomics sample preparation method that increased the sensitivity and reproducibility of large-scale plasma samples. Peptide loss was reduced while sensitivity was increased by eliminating the peptide clean-up step and using disposable trap column tips. The simplified High-Throughput Plasma Proteomics (sHTPP) method was evaluated using a systemic approach. We identified and quantified more than 500 proteins in non-depleted plasma samples without missing values as the DIA approach improved in terms of identification and quantification. Furthermore, 96-well plate sample handling with a multi-channel pipet allows for high-throughput sample analysis. In a large-scale clinical trial with 300 plasma samples, we used the method to demonstrate robust and accurate quantifications.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:40.232.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jongmin Woo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 12:39:04	ID requested
⏵ 1	2023-11-14 08:44:43	announced

Woo J, Zhang Q, A Streamlined High-Throughput Plasma Proteomics Platform for Clinical Proteomics with Improved Proteome Coverage, Reproducibility, and Robustness. J Am Soc Mass Spectrom, 34(4):754-762(2023) [pubmed]

submitter keyword: sHTPP, simplified High-Throughput Plasma Proteomics

Qibin Zhang
contact affiliation	Department of Center for Translational Biomedical Research at University of North Carolina at Greensboro, Kannapolis, NC, 28087
contact email	q_zhang2@uncg.edu
lab head
Jongmin Woo
contact affiliation	The University of North Carolina at Greensboro
contact email	jongminw@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD038669

PRIDE project URI

• PRIDE
  1. PXD038669
     1. Label: PRIDE project
     2. Name: Method development for simplified High-Throughput Plasma Proteomics