<<< Full experiment listing

PXD038668

PXD038668 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Nrg1 enhancer binding proteins
DescriptionHyperglycemia is a risk factor for breast cancer-related morbidity and mortality. Hyperglycemia induces Neuregulin 1 (Nrg1) overexpression in breast cancer, which subsequently promotes tumor progression. However, molecular mechanisms underlying hyperglycemia-induced Nrg1 overexpression remain poorly understood. Here, We used DNA-protein pull-down using Nrg1 enhancer sequence as bait followed by LC/MS and identified RBPJ as a key component of the Nrg1 enhanceosome. We show that hyperglycemia causes active histone modifications at the Nrg1 enhancer, forming enhanceosome complexes where RBPJ, P300, and SETD1A are recruited to upregulate Nrg1 expression.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:58:33.632.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD038668
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChanghu Lee
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 12:29:46ID requested
12023-01-29 15:23:48announced
22023-11-14 08:58:34announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD038668;
Keyword List
submitter keyword: LC-MSMS,mouse, breast cancer cells, high glucose
Contact List
Jiyoung Park
contact affiliationDepartment of Biological Sciences, College of Information and Bioengineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea
contact emailjpark@unist.ac.kr
lab head
Changhu Lee
contact affiliationUNIST
contact emailhulee@unist.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038668
PRIDE project URI
Repository Record List
[ + ]