PXD038643 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FIRE mimics a 14-3-3-binding motif to promote Phytophthora palmivora infection |
Description | The oomycete Phytophthora palmivora infects a wide range of tropical crops worldwide. Like other filamentous plant pathogens, it secretes effectors to colonise plant tissues. Here we characterise FIRE, an RXLR effector that contains a canonical mode I 14-3-3 phospho-sensor binding motif that is conserved in effectors of several Phytophthora species. FIRE is phosphorylated in planta and interacts with multiple 14-3-3 proteins. Binding is sensitive to the R18 14-3-3 inhibitor. FIRE promotes plant susceptibility and co-localises with its target around haustoria. This work uncovers a new type of oomycete effector target mechanism. It demonstrates that substrate mimicry for 14-3-3 proteins is a cross-kingdom effector strategy used by both prokaryotic and eukaryotic plant pathogens to suppress host immunity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_07:00:08.769.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD038643 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Edouard Evangelisti |
SpeciesList | scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-07 07:59:54 | ID requested | |
1 | 2024-10-01 01:25:12 | announced | |
⏵ 2 | 2024-10-22 07:00:09 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: biotrophy, effector, phosphorylation,Phytophthora, susceptibility |
Contact List
Sebastian Schornack |
contact affiliation | Sainsbury Laboratory Cambridge University (SLCU) |
contact email | sebastian.schornack@slcu.cam.ac.uk |
lab head | |
Edouard Evangelisti |
contact affiliation | Wageningen University & Research |
contact email | edouard.evangelisti@wur.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038643
- Label: PRIDE project
- Name: FIRE mimics a 14-3-3-binding motif to promote Phytophthora palmivora infection