PXD038643

PXD038643 is an original dataset announced via ProteomeXchange.

Title	FIRE mimics a 14-3-3-binding motif to promote Phytophthora palmivora infection
Description	The oomycete Phytophthora palmivora infects a wide range of tropical crops worldwide. Like other filamentous plant pathogens, it secretes effectors to colonise plant tissues. Here we characterise FIRE, an RXLR effector that contains a canonical mode I 14-3-3 phospho-sensor binding motif that is conserved in effectors of several Phytophthora species. FIRE is phosphorylated in planta and interacts with multiple 14-3-3 proteins. Binding is sensitive to the R18 14-3-3 inhibitor. FIRE promotes plant susceptibility and co-localises with its target around haustoria. This work uncovers a new type of oomycete effector target mechanism. It demonstrates that substrate mimicry for 14-3-3 proteins is a cross-kingdom effector strategy used by both prokaryotic and eukaryotic plant pathogens to suppress host immunity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:08.769.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038643
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Edouard Evangelisti
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 07:59:54	ID requested
1	2024-10-01 01:25:12	announced
⏵ 2	2024-10-22 07:00:09	announced	2024-10-22: Updated project metadata.

10.1094/MPMI-12-22-0251-R;

10.6019/PXD038643;

submitter keyword: biotrophy, effector, phosphorylation,Phytophthora, susceptibility

Sebastian Schornack
contact affiliation	Sainsbury Laboratory Cambridge University (SLCU)
contact email	sebastian.schornack@slcu.cam.ac.uk
lab head
Edouard Evangelisti
contact affiliation	Wageningen University & Research
contact email	edouard.evangelisti@wur.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038643
     1. Label: PRIDE project
     2. Name: FIRE mimics a 14-3-3-binding motif to promote Phytophthora palmivora infection