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PXD038619

PXD038619 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA multi-parameter optimization in middle-down analysis of monoclonal antibodies by LC-MS/MS
DescriptionIn antibody-based drug research, regulatory agencies request a complete characterization of antibody proteoforms covering both the amino acid sequence and all post-translational modifications. The usual mass spectrometry-based approach to achieve this goal is bottom-up proteomics, which relies on the digestion of antibodies, but does not allow the diversity of proteoforms to be assessed. Middle-down and top-down approaches have recently emerged as attractive alternatives but are not yet mastered and thus used in routine by many analytical chemistry laboratories. The work described here aims at providing guidelines to achieve the best sequence coverage for the fragmentation of intact light and heavy chains generated from a simple reduction of intact antibodies using Orbitrap mass spectrometry. Three parameters were found crucial to this aim: the use of an electron-based activation technique, the multiplex selection of precursor ions of different charge states and the combination of replicates.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:59:22.110.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathan Dhenin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 06:02:25ID requested
12023-03-15 03:28:09announced
22023-11-14 08:59:22announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Orbitrap MS, middle-down proteomics,monoclonal antibody, intact proteins, LC-MS/MS
Contact List
Julia Chamot-Rooke
contact affiliationMass Spectrometry for Biology Unit, Université Paris Cité, Institut Pasteur, CNRS UAR2024, Paris, France
contact emailjulia.chamot-rooke@pasteur.fr
lab head
Jonathan Dhenin
contact affiliationMass Spectrometry for Biology Unit Institut Pasteur Paris
contact emailjonathan.dhenin@pasteur.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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