PXD038619 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A multi-parameter optimization in middle-down analysis of monoclonal antibodies by LC-MS/MS |
Description | In antibody-based drug research, regulatory agencies request a complete characterization of antibody proteoforms covering both the amino acid sequence and all post-translational modifications. The usual mass spectrometry-based approach to achieve this goal is bottom-up proteomics, which relies on the digestion of antibodies, but does not allow the diversity of proteoforms to be assessed. Middle-down and top-down approaches have recently emerged as attractive alternatives but are not yet mastered and thus used in routine by many analytical chemistry laboratories. The work described here aims at providing guidelines to achieve the best sequence coverage for the fragmentation of intact light and heavy chains generated from a simple reduction of intact antibodies using Orbitrap mass spectrometry. Three parameters were found crucial to this aim: the use of an electron-based activation technique, the multiplex selection of precursor ions of different charge states and the combination of replicates. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:59:22.110.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonathan Dhenin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-07 06:02:25 | ID requested | |
1 | 2023-03-15 03:28:09 | announced | |
⏵ 2 | 2023-11-14 08:59:22 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Orbitrap MS, middle-down proteomics,monoclonal antibody, intact proteins, LC-MS/MS |
Contact List
Julia Chamot-Rooke |
contact affiliation | Mass Spectrometry for Biology Unit, Université Paris Cité, Institut Pasteur, CNRS UAR2024, Paris, France |
contact email | julia.chamot-rooke@pasteur.fr |
lab head | |
Jonathan Dhenin |
contact affiliation | Mass Spectrometry for Biology Unit Institut Pasteur Paris |
contact email | jonathan.dhenin@pasteur.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038619 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038619
- Label: PRIDE project
- Name: A multi-parameter optimization in middle-down analysis of monoclonal antibodies by LC-MS/MS