PXD038616 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The differential proteomic response of Pseudomonas aeruginosa and Staphylococcus aureus to the silver (1) compound, SBC3 |
Description | The race to combat antibiotic resistance and develop novel therapies has triggered studies on novel metal-based formulations. Silver remains a strong candidate since ancient times due to its multimodal and broad-spectrum activity against bacterial and fungal pathogens. N-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer and/or antimicrobial agents with ongoing efforts being made to enhance lipophilicity and drug stability. The lead silver(I) acetate complex, 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*) (SBC3) synthesised by the Tacke group has previously demonstrated promising growth and biofilm-inhibiting properties. As an extension of this, we examined the responses of two structurally different bacteria to SBC3 using label-free quantitative proteomic analysis. Multidrug resistant Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) are associated with chronic wound infections and Cystic Fibrosis lung colonisation where co-infection often exacerbates disease. SBC3 increased the abundance of alginate biosynthesis, secretion system and drug detoxification proteins in P. aeruginosa whilst a multitude of pathways including anaerobic respiration, twitching motility, and ABC transport were decreased. This contrasted with affected pathways in S. aureus such as increased DNA replication/repair and cell redox homeostasis and decreased protein synthesis, lipoylation, glucose metabolism. Increased abundance of cell wall/membrane proteins were indicative of the structural damage induced by SBC3 to both cell types. These findings show the potential broad applications of SBC3 in treating Gram-positive and Gram-negative bacteria. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:43:31.379.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Magdalena Piatek |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Pseudomonas aeruginosa PAO1LM10; NCBI TaxID: 990346; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-07 06:01:19 | ID requested | |
1 | 2023-03-11 10:31:36 | announced | |
⏵ 2 | 2023-11-14 08:43:38 | announced | 2023-11-14: Updated project metadata. |
Publication List
Piatek M, O'Beirne C, Beato Z, Tacke M, Kavanagh K, Display Differential Proteomic Responses to the Silver(I) Compound, SBC3. Antibiotics (Basel), 12(2):(2023) [pubmed] |
Keyword List
submitter keyword: Pseudomonas, Staphylococcus,Proteomics, Silver, Antimicrobial, LC-MS/MS |
Contact List
Professor Kevin Kavanagh |
contact affiliation | Biology Department, Maynooth University |
contact email | kevin.kavanagh@mu.ie |
lab head | |
Magdalena Piatek |
contact affiliation | Maynooth University |
contact email | magdalena.piatek@mu.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038616
- Label: PRIDE project
- Name: The differential proteomic response of Pseudomonas aeruginosa and Staphylococcus aureus to the silver (1) compound, SBC3