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PXD038616

PXD038616 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe differential proteomic response of Pseudomonas aeruginosa and Staphylococcus aureus to the silver (1) compound, SBC3
DescriptionThe race to combat antibiotic resistance and develop novel therapies has triggered studies on novel metal-based formulations. Silver remains a strong candidate since ancient times due to its multimodal and broad-spectrum activity against bacterial and fungal pathogens. N-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer and/or antimicrobial agents with ongoing efforts being made to enhance lipophilicity and drug stability. The lead silver(I) acetate complex, 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*) (SBC3) synthesised by the Tacke group has previously demonstrated promising growth and biofilm-inhibiting properties. As an extension of this, we examined the responses of two structurally different bacteria to SBC3 using label-free quantitative proteomic analysis. Multidrug resistant Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) are associated with chronic wound infections and Cystic Fibrosis lung colonisation where co-infection often exacerbates disease. SBC3 increased the abundance of alginate biosynthesis, secretion system and drug detoxification proteins in P. aeruginosa whilst a multitude of pathways including anaerobic respiration, twitching motility, and ABC transport were decreased. This contrasted with affected pathways in S. aureus such as increased DNA replication/repair and cell redox homeostasis and decreased protein synthesis, lipoylation, glucose metabolism. Increased abundance of cell wall/membrane proteins were indicative of the structural damage induced by SBC3 to both cell types. These findings show the potential broad applications of SBC3 in treating Gram-positive and Gram-negative bacteria.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:43:31.379.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMagdalena Piatek
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Pseudomonas aeruginosa PAO1LM10; NCBI TaxID: 990346;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 06:01:19ID requested
12023-03-11 10:31:36announced
22023-11-14 08:43:38announced2023-11-14: Updated project metadata.
Publication List
Piatek M, O'Beirne C, Beato Z, Tacke M, Kavanagh K, Display Differential Proteomic Responses to the Silver(I) Compound, SBC3. Antibiotics (Basel), 12(2):(2023) [pubmed]
Keyword List
submitter keyword: Pseudomonas, Staphylococcus,Proteomics, Silver, Antimicrobial, LC-MS/MS
Contact List
Professor Kevin Kavanagh
contact affiliationBiology Department, Maynooth University
contact emailkevin.kavanagh@mu.ie
lab head
Magdalena Piatek
contact affiliationMaynooth University
contact emailmagdalena.piatek@mu.ie
dataset submitter
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Dataset FTP location
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