PXD038611

PXD038611 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of the effects of Grammatophyllum speciosum extracts on RAW 264.7 cells
Description	Grammatophyllum speciosum is a traditional plant with beneficial functionalities for health. G. speciosum extracts can inhibit collagenase and nitric oxide without cellular toxicity in keratinocytes. The extracts have shown potential for use and formulation as cosmeceutical ingredients. However, the molecular mechanisms underlying these activities remain unknown. In this dataset, we used a proteomics approach to clarify the proteins that participate in the response of RAW264.7 macrophage cells to G. speciosum extracts. Cells were divided into two experimental groups, i.e., the control and treatment groups. In turn, the treatment group included two subgroups that were treated with 20 and 100 g/mL of the extracts, respectively. The experiments were conducted using two biological replicates. The dataset was obtained from label-free proteomics using high-resolution LC-MS/MS with four technical replicates. The quality control (QC) of the proteomics dataset was carried out using chromatography at the MS1 and MS2 levels, peptide mass deviation, peptide mass cleavage, sequence length, and total peptide intensity.
HostingRepository	PRIDE
AnnounceDate	2022-12-27
AnnouncementXML	Submission_2022-12-26_23:45:59.533.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SucheewinKrobthong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 05:30:31	ID requested
⏵ 1	2022-12-26 23:45:59	announced

10.6019/PXD038611;

submitter keyword: PCA, quality control,macrophage,traditional plant, LC-MS/MS

SucheewinKrobthong
contact affiliation	Department of Chemistry, Faculty of Science, Chulalongkorn University, Thailand
contact email	sucheewin82@gmail.com
lab head
SucheewinKrobthong
contact affiliation	Mahidol University
contact email	sucheewin82@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038611
     1. Label: PRIDE project
     2. Name: Proteomics analysis of the effects of Grammatophyllum speciosum extracts on RAW 264.7 cells