PXD038609

PXD038609 is an original dataset announced via ProteomeXchange.

Title	Multiomic approaches reveal hormonal modulation and nitrogen uptake and assimilation on the initial growth of maize inoculated with Herbaspirillum seropedicae
Description	Herbaspirillum seropedicae is an endophytic bacterium that can fix nitrogen and promote a hormonal imbalance that leads to a plant growth-promoting effect when used as a microbial inoculant. Studies focused on mechanisms of action are crucial for a better understanding of the bacteria-plant interaction and optimization of plant growth-promoting response. The work aims to understand the underlined mechanisms responsible for the early stimulatory growth effects of the H. seropedicae inoculation in maize. To perform it, we combined transcriptomic and proteomic approaches with physiological analysis. The results obtained with the inoculation showed increased root biomass (233 and 253%) and shoot biomass (249 and 264%), respectively, for the fresh and dry mass of maize seedlings and increased green content and development. Omics data analysis for the positive biostimulation phenotype revealed that inoculation increases N-uptake and N-assimilation machinery through differential expressed nitrate transporters and amino acids pathway, as well carbon/nitrogen metabolism integration by the tricarboxylic acid cycle and the polyamines pathway. Additionally, phytohormone levels of root and shoot tissues increased in bacterium-inoculated-maize plants leading to feedback regulation by the ubiquitin-proteasome system. The early biostimulatory effect of H. seropedicae partially results from hormonal imbalance coupled with efficient nutrient uptake-assimilation and a boost in primary anabolic metabolism of carbon-nitrogen integrative pathways.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:57.020.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabio Olivares
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 05:30:07	ID requested
1	2023-03-11 11:24:55	announced
⏵ 2	2023-11-14 08:44:00	announced	2023-11-14: Updated project metadata.

Irineu LESDS, Soares CP, Soares TS, Almeida FA, Almeida-Silva F, Gazara RK, Meneses CHSG, Canellas LP, Silveira V, Venancio TM, Olivares FL, . Plants (Basel), 12(1):(2022) [pubmed]

submitter keyword: endophytic bacteria

biostimulation

Herbaspirillum spp.

diazotrophs

plant-growth promotion

biofertilizer

Fabio Lopes Olivares
contact affiliation	Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil
contact email	fabioliv@uenf.br
lab head
Fabio Olivares
contact affiliation	UENF
contact email	fabioliv@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038609

PRIDE project URI

• PRIDE
  1. PXD038609
     1. Label: PRIDE project
     2. Name: Multiomic approaches reveal hormonal modulation and nitrogen uptake and assimilation on the initial growth of maize inoculated with Herbaspirillum seropedicae