PXD038603

PXD038603 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Profiling of early Neoplastic Gastric Microen-vironment Modifications and Biodynamics in Impaired BMP-Signaling Foxl1+-Telocytes
Description	FoxL1+telocytes (TCFoxL1+) are novel gastrointestinal subepithelial cells that form communication axis between the mesenchyme and epithelium. TCFoxL1+ are strategically positioned to be key contributors to the microenvironment through production and secretion of growth factors and extracellular matrix (ECM) proteins. In recent years, the alteration of the bone morphogenetic protein (BMP) signaling in TCFoxL1+ was demonstrated to trigger a toxic microenvironment with ECM remodeling that leads to the development of pre-neoplastic gastric lesions. However, a comprehensive analysis of variations in the ECM composition and its associated proteins in gas-tric neoplasia linked to TCFoxL1+ dysregulation has never been performed. This study provides a better understanding of how TCFoxL1+ defective BMP signaling participates in gastric pre-neoplastic microenvironment. Using a proteomic approach, we determined the changes in the complete matrisome of BmpR1a△FoxL1+ and control mice, both in total antrum as well as in isolated mesenchyme-enriched antrum fractions. Comparative proteomic analysis revealed that the deconstruction of the gastric antrum led to a more comprehensive analysis of the ECM frac-tion of gastric tissues microenvironment. These results show that TCFoxL1+ are key members of the mesenchymal cell population and actively participate in the establishment of the matrisomic fraction of the microenvironment; thus, influencing epithelial cell behavior
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:07.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Levesque
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 03:34:00	ID requested
1	2023-03-11 11:55:35	announced
⏵ 2	2023-11-14 08:44:15	announced	2023-11-14: Updated project metadata.

Alfonso AB, Pomerleau V, Nicol, á, s VR, Raisch J, Jurkovic CM, Boisvert FM, Perreault N, -Telocytes. Biomedicines, 11(1):(2022) [pubmed]

submitter keyword: BMP signaling

Extracellular matrix

Me-chanical microenvironment

Matrisome,FoxL1+-Telocytes

Epithelial-mesenchymal interaction

Nathalie Perreault
contact affiliation	Département d’Immunologie et Biologie Cellulaire, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Québec, Canada,
contact email	Nathalie.perreault@USherbrooke.ca
lab head
Dominique Levesque
contact affiliation	University of Sherbrooke, FM Boisvert lab
contact email	dominique.levesque@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038603

PRIDE project URI

• PRIDE
  1. PXD038603
     1. Label: PRIDE project
     2. Name: Comprehensive Profiling of early Neoplastic Gastric Microen-vironment Modifications and Biodynamics in Impaired BMP-Signaling Foxl1+-Telocytes