PXD038596

PXD038596 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide Analysis of Lysine β-hydroxybutyrylation in the myocardium of diabetic rat model with cardiomyopathy
Description	Lysineβ-hydroxybutyrylation (kbhb), a novel protein post-translational modification (PTM) of lysine residues withβ-hydroxybuty group, is associated with ketone metabolism in numerous species. However, its potential role in diabetes, especially in diabetic cardiomyopathy (DCM), remains largely unexplored. In this study, we performed antibody-based affinity enrichment and liquid chromatography-mass spectrometry (LC-MS/MS) to analyze the kbhb residues on heart tissues of both wild types and established DCM model rat quantitatively. A total of 3520 β-hydroxybutyrated lysine sites in 1089 proteins were identified in this study. Further analysis showed that 336 kbhb sites in 143 proteins were differentially expressed between the heart tissues of DCM and wild-type rats. Among them, 284 Kbhb sites corresponding to 96 proteins increased, while 52 corresponding to 47 proteins decreased. Bioinformatic analysis of the proteomic results showed the up-regulated proteins in the heart tissues of DCM rats to be primarily located in the cytoplasm. In contrast, the down-regulated kbhb proteins were distributed mainly in the mitochondria. Functional enrichment analysis showed that the up-regulated proteins were enriched in the tricarboxylic acid cycle, oxidative phosphorylation, and propanoate metabolism. In contrast, the down-regulated proteins were enriched in the cGMP-PKG signaling pathway and glutathione metabolism. Our findings demonstrated how kbhb is related to many metabolic pathways, especially in those involved in energy metabolism. This study provides the first global investigation of the kbhb profile in DCM progression and can be an essential resource to explore DCM's pathogenesis further.
HostingRepository	iProX
AnnounceDate	2022-12-07
AnnouncementXML	Submission_2023-01-29_19:49:56.031.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weiguang Luo
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	butanoylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-06 18:57:23	ID requested
1	2022-12-06 18:57:36	announced
⏵ 2	2023-01-29 19:49:56	announced	2023-01-30: Update publication information.

Luo W, He M, Luo Q, Li Y, -hydroxybutyrylation in the myocardium of diabetic rat model with cardiomyopathy. Front Cardiovasc Med, 9():1066822(2022) [pubmed]

submitter keyword: Lysine β-hydroxybutyrylation, Post-translational modification, Proteomics, Liquid chromatography-mass spectrometry, Diabetic cardiomyopathy

Weiguang Luo
contact affiliation	Henan Provincial People's Hospital
contact email	1341154329@QQ.com
lab head
Weiguang Luo
contact affiliation	Henan Provincial People's Hospital
contact email	weiguangluo@126.com
dataset submitter

iProX dataset URI