PXD038583

PXD038583 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mitochondrial translocase blocking ATP5MG-tFT fusion protein interactome in human embryonic kidney (HEK) 293 cells
Description	The experiment uses HEK-293 Flp-In T-Rex cells to express fusion protein that combines ATP synthase subunit ATP5MG with a tandem fluorescent protein timer (tFT). The tFT tag combines mCherry and super folder GFP (sfGFP) fluorescent proteins. An exceptionally stable structure of sfGFP interferes with its import into mitochondria. Thus, ATP5MG-tFT fusion stalls during translocation into mitochondria. We identify the proteome of cells after 24 hours of fusion protein expression. As a control, we use cells that express tFT fusion not directed to mitochondria (ILE-Deg-tFT) or cells that did not express fusion protein. Next, we use chemical crosslinking and GFP-Trap-based immunoprecipitation to define interaction partner proteins of ATP5MG-tFT and ILE-Deg-tFT fusions.
HostingRepository	PRIDE
AnnounceDate	2024-03-08
AnnouncementXML	Submission_2024-03-08_03:57:39.301.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-06 07:19:30	ID requested
⏵ 1	2024-03-08 03:57:39	announced

Publication List

10.1083/JCB.202306051;

10.1083/JCB.202306051;

Keyword List

submitter keyword: translocase of the outer mitochondrial membrane,Protein import, quality control, mitochondria, translocation

submitter keyword: translocase of the outer mitochondrial membrane,Protein import, quality control, mitochondria, translocation

Contact List

Dr Piotr Bragoszewski
contact affiliation	Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland
contact email	p.bragoszewski@nencki.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD038583
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD038583

PRIDE project URI

Repository Record List

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