PXD038552

PXD038552 is an original dataset announced via ProteomeXchange.

Title	Retinal proteome analysis reveals region-specific change in the rabbit myopia model
Description	Uncovering region-specific change in myopic retina can provide the clue for explaining the pathogenesis of myopia progression. After imposing form deprivation myopia (FDM) on the right eye of 6-week-old rabbits, we investigated the proteome profile of each retinal region (central, mid periphery, and far periphery retina) using high-resolution, accurate mass (HRAM) mass spectrometry. Protein expression was analyzed with gene ontology and network analysis compared to the control, the left eyes. Among total 2065 proteins detected from whole retinal samples, 249 differentially-expressed proteins (DEPs) were identified: 164 DEPs in far periphery; 39 DEPs in mid periphery; 83 DEPs in central retina. In network analysis, far periphery retina showed the most significant connectivity between DEPs. Regulation of coagulation was the most significant biological process in up-regulated DEPs in far periphery retina. Proteasome was the most significant KEGG pathway in down-regulated DEPs in central retina. Antithrombin-III, fibrinogen gamma chain, and fibrinogen beta chain were identified as biomarkers for myopia progression, which were up-regulated in far periphery retina. Proteomic analysis in this study suggested that the oxidative stress can be the main pathogenesis of myopia progression and the far periphery retina played a role as the key responder.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:34:20.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chae Eun Moon
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-05 06:24:36	ID requested
1	2023-03-10 12:19:49	announced
⏵ 2	2023-11-14 08:34:21	announced	2023-11-14: Updated project metadata.

Moon CE, Ji YW, Lee JK, Han K, Kim H, Byeon SH, Han S, Han J, Seo Y, Retinal Proteome Analysis Reveals a Region-Specific Change in the Rabbit Myopia Model. Int J Mol Sci, 24(2):(2023) [pubmed]

submitter keyword: Retina, Rabbit,Myopia, LC-MS/MS

Yuri Seo
contact affiliation	Department of Ophthalmology, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Korea
contact email	ophyrseo@yonsei.ac.kr
lab head
Chae Eun Moon
contact affiliation	Yonsei University College of Medicine
contact email	cemoon@yuhs.ac
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038552

PRIDE project URI

• PRIDE
  1. PXD038552
     1. Label: PRIDE project
     2. Name: Retinal proteome analysis reveals region-specific change in the rabbit myopia model