PXD038550

PXD038550 is an original dataset announced via ProteomeXchange.

Title	A unique mRNA decapping complex in trypanosomes
Description	Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2 orthologues but instead rely on the ApaH-like phosphatase ALPH1 for decapping. The enzyme is composed of a catalytic domain flanked by C-terminal and N-terminal extensions. Here we analyse the ALPH1 interactome by BioID proximity labelling for the full length protein and truncated versions in order to assign domain specific interactions. We show that Trypanosoma brucei ALPH1 acts in a complex composed of the trypanosome XRN1 ortholog XRNA and four proteins that are unique to Kinetoplastida.The interactome is validated by reverse experiments targeting T. brucei and T. cruzi XRNA by affinity capture and, additionally, the ALPH1 interacting CMGC-family kinase by BioID.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:48.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-05 05:10:06	ID requested
1	2023-06-14 06:23:10	announced
⏵ 2	2023-11-14 09:00:49	announced	2023-11-14: Updated project metadata.

Kramer S, Karolak NK, Odenwald J, Gabiatti B, Casta, ñ, eda Londo, ñ, o PA, Zav, ř, elov, á A, Freire ER, Almeida KS, Braune S, Moreira C, Eder A, Goos C, Field M, Carrington M, Holetz F, G, ó, rna MW, Zoltner M, A unique mRNA decapping complex in trypanosomes. Nucleic Acids Res, 51(14):7520-7540(2023) [pubmed]

submitter keyword: XRNA, mRNA decay, ApaH-like phosphatase, ALPH1,mRNA decapping, Trypanosoma, exoribonuclease

Martin Zoltner
contact affiliation	Head of Drug Discovery and Evaluation Center for Research of Pathogenicity and Virulence of Parasites Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic
contact email	zoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038550
     1. Label: PRIDE project
     2. Name: A unique mRNA decapping complex in trypanosomes