Updated project metadata. Ginseng is an important crop in East Asia due to its medicinal and nutritional benefits originating from ingredients such as the ginsenosides. Numerous researches have been directed to cultivate ginseng with high yield especially targeting its growth and development for protection against abiotic stresses, which are affecting both the yield and quality. Particularly, salinity has been characterized as a major abiotic stressor that affects the annual yield of ginseng. Therefore, to characterize the salt-responsive proteins in the ginseng plant, ginseng leaves were harvested post-treatment with salt in a time-dependent manner. Utilizing a label-free quantitative proteome analysis approach, this study identified a total of 2,484 proteins. Among them, 468 proteins showed a significant modulation in their abundance among ginseng leaf samples at 4 different time points (0, 24, 72, 96 h) following salt stress. Further functional classification revealed that catalase-peroxidase 2, voltage-gated potassium channel subunit beta-2, fructose-1,6-bisphosphatase class 1, and chlorophyll a-b binding protein associated proteins accumulated in response to the salt stress. Of these, glycosyl hydrolase 17 (GH17) showed similar abundance profiles at both the transcript and proteome level. Therefore, for further understanding of GH17 underlying salt stress response mechanism, GH17 overexpressing transgenic Arabidopsis were generated. In response to salt stress, transgenic plants uncovered a tolerance phenotype without compromising plant growth. The proteome alterations in response to salt stress presented here resulted in identifying a protein GH17 with a potential key role in salt-stressed ginseng.