PXD038521 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Kansl2 dependent acetylome study in primary Kansl2 (fl/fl) murine embryonic fibroblasts |
Description | As one of the most important post-translational protein modifications, lysine acetylation is catalyzed by lysine acetyltransferases (KATs), which catalyze the covalent attachment of an acetyl group to the N epsilon–residue of lysine. The histone acetyltransferase MOF, which represents the main histone H4 lysine-16 specific acetyltransferase, is the KAT subunit of two mutually exclusive multiprotein complexes in metazoa, namely the MSL (male-specific lethal) and the NSL (non-specific lethal) complex. Depletion of both, MOF and the NSL complex subunit Kansl2 result in selective changes of the cellular acetylome. As a consequence, modulation of nuclear lamin lysine acetylations correlate with profound changes in nuclear morphology, like micronuclei formation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:29:11.160.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gerhard Mittler |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-02 17:57:21 | ID requested | |
1 | 2023-01-05 04:38:12 | announced | |
⏵ 2 | 2023-11-14 08:29:12 | announced | 2023-11-14: Updated project metadata. |
Publication List
Karoutas A, Szymanski W, Rausch T, Guhathakurta S, Rog-Zielinska EA, Peyronnet R, Seyfferth J, Chen HR, de Leeuw R, Herquel B, Kimura H, Mittler G, Kohl P, Medalia O, Korbel JO, Akhtar A, The NSL complex maintains nuclear architecture stability via lamin A/C acetylation. Nat Cell Biol, 21(10):1248-1260(2019) [pubmed] |
Keyword List
submitter keyword: KAT8 regulatory NSL complex subunit 2 |
MEF (murine embryonic fibroblast) |
primary inducible Kansl2 knock-out |
Cre-ERT2 |
anti acetyl-lysine antibody |
PTM enrichment; |
Contact List
Gerhard Mittler |
contact affiliation | Proteomics Unit Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
lab head | |
Gerhard Mittler |
contact affiliation | Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038521 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038521
- Label: PRIDE project
- Name: Kansl2 dependent acetylome study in primary Kansl2 (fl/fl) murine embryonic fibroblasts