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PXD038521

PXD038521 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleKansl2 dependent acetylome study in primary Kansl2 (fl/fl) murine embryonic fibroblasts
DescriptionAs one of the most important post-translational protein modifications, lysine acetylation is catalyzed by lysine acetyltransferases (KATs), which catalyze the covalent attachment of an acetyl group to the N epsilon–residue of lysine. The histone acetyltransferase MOF, which represents the main histone H4 lysine-16 specific acetyltransferase, is the KAT subunit of two mutually exclusive multiprotein complexes in metazoa, namely the MSL (male-specific lethal) and the NSL (non-specific lethal) complex. Depletion of both, MOF and the NSL complex subunit Kansl2 result in selective changes of the cellular acetylome. As a consequence, modulation of nuclear lamin lysine acetylations correlate with profound changes in nuclear morphology, like micronuclei formation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:29:11.160.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGerhard Mittler
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-02 17:57:21ID requested
12023-01-05 04:38:12announced
22023-11-14 08:29:12announced2023-11-14: Updated project metadata.
Publication List
Karoutas A, Szymanski W, Rausch T, Guhathakurta S, Rog-Zielinska EA, Peyronnet R, Seyfferth J, Chen HR, de Leeuw R, Herquel B, Kimura H, Mittler G, Kohl P, Medalia O, Korbel JO, Akhtar A, The NSL complex maintains nuclear architecture stability via lamin A/C acetylation. Nat Cell Biol, 21(10):1248-1260(2019) [pubmed]
Keyword List
submitter keyword: KAT8 regulatory NSL complex subunit 2
MEF (murine embryonic fibroblast)
primary inducible Kansl2 knock-out
Cre-ERT2
anti acetyl-lysine antibody
PTM enrichment;
Contact List
Gerhard Mittler
contact affiliationProteomics Unit Max Planck Institute of Immunobiology and Epigenetics
contact emailmittler@ie-freiburg.mpg.de
lab head
Gerhard Mittler
contact affiliationProteomics Unit, Max Planck Institute of Immunobiology and Epigenetics
contact emailmittler@ie-freiburg.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038521
PRIDE project URI
Repository Record List
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