PXD038503

PXD038503 is an original dataset announced via ProteomeXchange.

Title	A degron blocking strategy towards improved CRBN recruiting PROTAC selectivity
Description	The different layers of complexity in PROTAC design have slowed the broad implementation of this novel pharmacological approach. Given the challenges and opportunities of CRL4CRBN hijacking PROTACs we set out to identify and develop broadly implementable strategies to generate PROTACs with improved specificity for their targets. We used structural analysis of known neo-substrate degrons to generate “degron blocked IMiD” analogues and validated that they did not degrade any known neo-substrates using quantitative proteomics. We then applied this strategy to a known BRD9 PROTAC (dBRD9-A) to generate a degron-blocking analogue with a specific degradation profile.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:02.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernando J. Sialana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-01 16:42:55	ID requested
1	2023-07-10 09:55:37	announced
⏵ 2	2023-11-14 09:02:10	announced	2023-11-14: Updated project metadata.

Bouguenina H, Scarpino A, O'Hanlon JA, Warne J, Wang HZ, Wah Hak LC, Sadok A, McAndrew PC, Stubbs M, Pierrat OA, Hahner T, Cabry MP, Le Bihan YV, Mitsopoulos C, Sialana FJ, Roumeliotis TI, Burke R, van Montfort RLM, Choudhari J, Chopra R, Caldwell JJ, Collins I, Recruiting PROTAC Selectivity. Chembiochem, 24(23):e202300351(2023) [pubmed]

submitter keyword: cereblon,PROTACs

Jyoti S. Choudhary
contact affiliation	Institute of Cancer Research, London
contact email	jyoti.choudhary@icr.ac.uk
lab head
Fernando J. Sialana
contact affiliation	Institute of Cancer Research
contact email	fsialana@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD038503

PRIDE project URI

• PRIDE
  1. PXD038503
     1. Label: PRIDE project
     2. Name: A degron blocking strategy towards improved CRBN recruiting PROTAC selectivity